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一株产碳酸酐酶附生菌对铜绿微囊藻(Microcystis aeruginosa)生长的影响
引用本文:邓洁,李建宏,管章玲,胡碧洋,赵蕾,李朋富.一株产碳酸酐酶附生菌对铜绿微囊藻(Microcystis aeruginosa)生长的影响[J].湖泊科学,2012,24(3):429-435.
作者姓名:邓洁  李建宏  管章玲  胡碧洋  赵蕾  李朋富
作者单位:1. 南京师范大学生命科学学院,南京,210046
2. 南京大学生命科学学院,南京,210093
基金项目:国家重点基础研究发展计划项目,国家基础科学人才培养基金,江苏高校优势学科建设工程项目联合资助
摘    要:为研究生长环境中微生物对铜绿微囊藻碳代谢的影响,本文分析太湖典型微囊藻水华样品附生菌中产碳酸酐酶(CA)细菌的比例,结果显示CA菌占11.6%;从微囊藻群体中分离获得了一株高胞外CA附生菌P201,通过ITS基因鉴定,该菌为一株荧光假单胞菌(Pseudomonas fluorescence).并研究了该菌在不同浓度HCO3-条件下对铜绿微囊藻生长的影响,结果表明无论是高HCO3-浓度还是低HCO3-浓度环境中,加入该菌对铜绿微囊藻的生长均有促进作用,说明产CA酶附生菌对铜绿微囊藻的生长有一定的促进作用.

关 键 词:铜绿微囊藻  荧光假单胞菌  附生菌  碳酸酐酶
收稿时间:2011/7/15 0:00:00
修稿时间:2011/12/2 0:00:00

Effect of attached bacteria of carbonic anhydrase on the growth of Microcystis aeruginosa
DENG Jie,LI Jianhong,GUAN Zhangling,HU Biyang,ZHAO Lei and LI Pengfu.Effect of attached bacteria of carbonic anhydrase on the growth of Microcystis aeruginosa[J].Journal of Lake Science,2012,24(3):429-435.
Authors:DENG Jie  LI Jianhong  GUAN Zhangling  HU Biyang  ZHAO Lei and LI Pengfu
Institution:1: Life Sciences College of Nanjing Normal University,Nanjing 210046,P.R.China)(2: School of Life Science,Nanjing University,Nanjing 210093,P.R.China)
Abstract:To study the effect of attached bacteria on the carbon metabolism of M.aeruginosa,the percentage of carbonic anhydrase(CA) produced bacteria in total attached bacteria was analyzed.The result showed that the ratio of CA produced bacteria is 11.6% in a typical cyanobacterial bloom from Lake Taihu.An attached bacterium strain P201,which produced large amount of extracellular carbonic anhydrase,was isolated from M.aeruginosa assemblage.It was identified as a strain of Pseudomonas fluorescence by its ITS molecular characteristic.Effects of P201 on the growth of M.aeruginosa in different HCO-3 concentrations were investigated.The results showed that no matter in the higher or the lower HCO-3 culture medium,P201 could promote the growth of M.aeruginosa,which suggested that CA produced attached bacteria could enhance the growth of M.aeruginosa by regulating the ambient carbon environment.
Keywords:Microcystis aeruginosa  Pseudomonas fluorescence  attached bacteria  carbonic anhydrase
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