| 罗氏沼虾胸神经节中促进卵母细胞发育的激素的初步分离 |
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| 引用本文： | 孙继贤,廖家遗.罗氏沼虾胸神经节中促进卵母细胞发育的激素的初步分离[J].湖泊科学,2003,15(1):64-69. |
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| 作者姓名： | 孙继贤 廖家遗 |
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| 作者单位： | 中山大学生命科学学院,广州,510275 |
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| 基金项目： | 国家自然科学基金资助项目30070105. |
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| 摘 要： | 罗氏沼虾成虾的胸神经节匀浆经Sephadex-G50柱层析所得第2个峰再经HPLC分离得到9个组分. 第1个组分和罗氏沼虾期卵巢小块一起体外培养36h可使卵母细胞的直径较对照组非常显著地增大P0.01这种增大卵母细胞直径的能力随该组分中蛋白质浓度的增加而增加. 在该组分中的激素分子量在1.3kDa左右. 结果显示罗氏沼虾胸神经节能分泌促进卵母细胞发育的激素.
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| 关 键 词： | 罗氏沼虾 卵母细胞 发育 成虾 大卵 激素 分离 分泌 柱层析 蛋白质浓度 |
| 收稿时间： | 2001/8/28 0:00:00 |
| 修稿时间： | 2001年8月28日 |
Preliminary Isolation of A Hormone Stimulating Oocyte Development from Thoracic Ganglion of the Prawn, Macrobrachium rosenbergii |
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| SUN Jixian and LIAO Jiayi.Preliminary Isolation of A Hormone Stimulating Oocyte Development from Thoracic Ganglion of the Prawn, Macrobrachium rosenbergii[J].Journal of Lake Science,2003,15(1):64-69. |
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| Authors: | SUN Jixian and LIAO Jiayi |
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| Institution: | School of Life Sciences, Zhongshan University, Guangzhou 510275, P. R. China and School of Life Sciences, Zhongshan University, Guangzhou 510275, P. R. China |
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| Abstract: | The extracts of thoracic ganglion from adult Macrobrachium rosenbergii were chromatographed on a Sephadex G-50 column, the resulting second peak was further isolated by HPLC, and 9 fractions were obtained. Among the fractions only the first fraction significantly increased the oocyte diameter in vitro of the stage ovary in M. rosenbergii. The increasing effects were depended on the protein concentration of the fraction for culture. The molecular weight of the substance in the fraction 1 was about 1.3kDa.The results show that the thoracic ganglion of M. rosenbergii can secrete peptide hormone which stimulates the oocyte development of the prawn. |
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| Keywords: | Macrobrachium rosenbergii thoracic ganglion oocyte development Hormone isolation |
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