| 滇池铜绿微囊藻(M. aeruginosa Kutz)的分离培养与总DNA提取的改进 |
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| 引用本文： | 陆源,文建凡,吕天雯.滇池铜绿微囊藻(M. aeruginosa Kutz)的分离培养与总DNA提取的改进[J].湖泊科学,2001,13(3):285-288. |
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| 作者姓名： | 陆源 文建凡 吕天雯 |
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| 作者单位： | 中国科学院昆明动物研究所 |
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| 基金项目： | 云南省中青年学术和技术带头人培养经费和云南省应用基础研究基金联合资助. |
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| 摘 要： | 利用稀释法结合平板分离培养法将采用滇池水华中的铜绿微囊灌进行了成功的分离纯化，对比纯化培养前后，发现纯化后该藻的生长速度明显加快，密度大为提高，约为纯化前的5倍，由乙醇乙醚混合液对该灌作预处理后，破坏了其胶质被，再经常规方法提取总DNA，其得率提高3－4倍。
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| 关 键 词： | 铜绿微囊藻 分离 总DNA提取 稀释法 技术改进 纯化培养 |
| 收稿时间： | 2000/12/18 0:00:00 |
| 修稿时间： | 2000年12月18 |
Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kutz in Dianchi Lake |
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| LU Yuan,WEN Jianfan and LV Tianwen.Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kutz in Dianchi Lake[J].Journal of Lake Science,2001,13(3):285-288. |
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| Authors: | LU Yuan WEN Jianfan and LV Tianwen |
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| Institution: | Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China,Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China and Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China |
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| Abstract: | M. aeruginosa Kütz is harmful to ecological envi ronmentof lakes.The algae in waterbloom of Dianchi Lake was isolated with methods of dilution and agar solid-media culture.It was found that after purificatio n, grow thing rate of the alg ae was raised by 5 times in liquid culture media.When extracting total DNA of the algae, if the cells were pre-treated by alcohol + ether (1:1), their gelatinous sheathes were broken and the production of total DNA increased by 3-4 times.The improvementof DNA ex traction is useful to molecular biology studies of the algae. |
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| Keywords: | M aeruginosa Kü tz pure cultivation total DNA ext raction |
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