| 我国五大湖三角帆蚌群体ITS-1序列变异分析 |
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| 引用本文： | 王建军,李家乐,汪桂玲,白志毅.我国五大湖三角帆蚌群体ITS-1序列变异分析[J].湖泊科学,2008,20(2):208-214. |
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| 作者姓名： | 王建军 李家乐 汪桂玲 白志毅 |
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| 作者单位： | 1. 上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海,200090
2. 上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海,200090;上海高校水产养殖学E-研究院,上海,200090 |
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| 基金项目： | 国家科技支撑计划
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上海市科委资助项目
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农业部农业结构调整技术研究专项基金
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上海市重点学科建设项目 |
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| 摘 要： | 对我国五大淡水湖:鄱阳湖、洞庭湖、太湖、巢湖、洪泽湖,鄱阳湖内包括进贤、余干、珠湖、都昌、湖口、永修,合计10个群体三角帆蚌78个个体核糖体DNA基因转录间隔子ITS-1进行PCR扩增、序列测定,获得78条长度为430 bp的同源序列.同源基因序列分析显示,五大淡水湖10个群体三角帆蚌78条ITS-1序列片段,G C的含量都明显高于A T的含量.都阳湖三角帆蚌的核苷酸多态性指数最高,而巢湖的核苷酸多样性指数最低.基于ITS-1序列片段的遗传距离表明,鄱阳湖内六个点群体间遗传距离很小,在0.071到0.0092之间.五大湖间三角帆蚌群体遗传距离较远,在0.0752到O.1381之间.ITS-1序列片段构建系统树显示,鄱阳湖6个群体聚在了一起为单独一支,并与巢湖群体亲缘关系相近.洞庭湖群体和洪泽湖群体亲缘关系相近,单独为一支.太湖群体从都阳湖、巢湖群体分离开来,单独为一支.
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| 关 键 词： | 三角帆蚌 转录间隔区 ITS-1 遗传多样性 亲缘关系 |
| 收稿时间： | 2007-03-07 |
| 修稿时间： | 2007-04-03 |
Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of Hyriopsis cumingii from the Five Freshwater Lakes of China |
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| WANG Jianjun,LI Jiale,WANG Guiling and BAI Zhiyi.Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of Hyriopsis cumingii from the Five Freshwater Lakes of China[J].Journal of Lake Science,2008,20(2):208-214. |
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| Authors: | WANG Jianjun LI Jiale WANG Guiling and BAI Zhiyi |
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| Institution: | 1;2;1:Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecology Certificated by the Ministry of Agriculture;Shanghai Fisheries University;Shanghai 200090;PR.China;2:Aquaculture Division;E-Institute of Shanghai Universities;P.R.China |
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| Abstract: | The internal transcribed spacer 1(ITS-1)was amplified from Hyriopsis cumingii in the five large lakes of China:Lake Poyang,Lake Dongting,Lake Taihu,Lake Chaohu and Lake Hongze,and 430bp lengths of homoiogical fragments of the ITS-1 was sequenced from 78 individuals of ten stocks.The homological fragment analysis showed that the average contents of G+C were obviously higher than that of A+T.The nucleotide diversity of ITS of Lake Poyang was the highest,while that of Lake Chaohu was the lowest.The genetic dis... |
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| Keywords: | Hyriopsis cumingii internal transcribed spacer ITS-1 genetic diversity phylogenetic relationship |
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