| 转基因鱼试验湖浮游生物群落DNA多态性与物种组成关系 |
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| 引用本文： | 李学梅,余育和,冯伟松,颜庆云,吴利,张翔.转基因鱼试验湖浮游生物群落DNA多态性与物种组成关系[J].湖泊科学,2009,21(3):375-381. |
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| 作者姓名： | 李学梅 余育和 冯伟松 颜庆云 吴利 张翔 |
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| 作者单位： | 1. 中国科学院水生生物研究所,武汉,430072;中国科学院研究生院,北京,100049
2. 中国科学院水生生物研究所,武汉,430072 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金 |
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| 摘 要： | 采用RAPD和PCR-DGGE指纹技术对转基因鱼试验湖的浮游生物群落DNA多态性进行了研究,并探讨了DNA多态性与物种组成的关系.结果显示:(1)形态学分类共鉴定到44种/类浮游生物:其中藻类13种,原生动物11种,轮虫16种,枝角类和桡足类各2种.多甲藻(Peridinium sp.)、球形砂壳虫(Difllugia globulosa)、螺形龟甲轮虫(Keratella cochlearis)和针簇多肢轮虫(Polyarthra trigla)4个物种在各个站丰度相对较高.(2)RAPD扩增共获得128条长度在200-1200bp的谱带,多态率为61.7%,特异性谱带占总谱带的19.5%.(3)PCR-DGGE指纹分析共获得87条扩增谱带,其中原核生物谱带相对较多(50条),真核生物谱带较少(37条),多态率分别为86%和64.9%.尽管形态学鉴定和DNA指纹分析都表现出较高物种多样性,但其相似性聚类却有差别:物种组成聚类中B、C站聚为一类,D、E站聚为一类,A站独为一类;两种DNA指纹分析聚类结果显示C、D、E三站聚为一类,A、B聚为一类.综上所述,在对浮游生物群落的研究中,形态学方法与DNA指纹技术不显著对应,后者能揭示更丰富的物种多样性,但毫无疑问三种方法可以从不同方面表征群落结构.这将为进一步研究湖泊浮游生物群落结构和功能关系奠定坚实的基础.
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| 关 键 词： | DNA多态性 浮游生物群落 转基因鱼试验湖 |
| 收稿时间： | 2008/7/29 0:00:00 |
| 修稿时间： | 2008/9/11 0:00:00 |
DNA polymorphism and species composition of plankton in an artificial lake for breeding transgenic fish |
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| LI Xuemei,YU Yuhe,FENG Weisong,YAN Qingyun,WU Li and ZHANG Xiang.DNA polymorphism and species composition of plankton in an artificial lake for breeding transgenic fish[J].Journal of Lake Science,2009,21(3):375-381. |
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| Authors: | LI Xuemei YU Yuhe FENG Weisong YAN Qingyun WU Li and ZHANG Xiang |
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| Institution: | 1: Institute of Hydrobiology;Chinese Academy of Sciences;Wuhan 430072;P.R.China;2: Graduate School of Chinese Academy of Sciences;Beijing 100049;P.R.China |
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| Abstract: | The present study was designed to explore relationship between DNA polymorphism, as revealed by RAPD and PCR-DGGE fingerprintings, and species composition of plankton community in an artificial lake for breeding transgenic fish. Planktonic samples were collected from five sampling sites in the artificial lake, and their similarity was assessed with cluster analysis. The results were: (1) a total of 44 planktonic taxa were identified, among which there were 13 algae, 11 protozoa, 16 rotifer, 2 Cladocera and ... |
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| Keywords: | RAPD PCR-DGGE |
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