| 浮游植物叶绿素a含量测定方法的比较测定 |
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| 引用本文： | 陈宇炜,高锡云.浮游植物叶绿素a含量测定方法的比较测定[J].湖泊科学,2000,12(2):185-188. |
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| 作者姓名： | 陈宇炜 高锡云 |
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| 作者单位： | 中国科学院南京地理与湖泊研究所南京210008 |
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| 基金项目： | 国家自然科学基金项目!( 3 960 0 0 2 5)，中国科学院重大项目!( KZ951 -B1 -2 0 5-0 2 )，中国科学院重点项目!( KZ952 -S1-2 2 0 ) |
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| 摘 要： | 本文比较研究了浮游植物叶绿素ａ含量测定的２种常用方法－国内常用的丙酮萃取分光光度法和国际上较通用的热乙萃取分光光度法。实验结果显示：热乙醇法具有操作简便、快捷、萃取完全，低毒等优点。两种方法有显著统计差异及很好的相关性，其回归方程为：Ｃｈｌａ乙醇＝１．２６１Ｃｈｌａ丙醇－３．５（Ｒ＝０．９９８）。
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| 关 键 词： | 浮游植物 叶绿素a 丙酮萃取法 热乙醇萃取法 |
| 收稿时间： | 7/2/1999 12:00:00 AM |
| 修稿时间： | 1999年7月2日 |
Comparison of Two Methods for Phytoplankton Chlorophyll-a ConcentrationMeasurement |
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| CHEN Yuwei and GAO Xiyun.Comparison of Two Methods for Phytoplankton Chlorophyll-a ConcentrationMeasurement[J].Journal of Lake Science,2000,12(2):185-188. |
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| Authors: | CHEN Yuwei and GAO Xiyun |
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| Institution: | Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China and Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China |
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| Abstract: | This study compared two simplified methods for phytoplankton chlorophyll a concentration measurement-the hot ethanol method which use 90% hot ethanol as the chlorophyll a extraction agent and the cool acetone method which use 90% cool acetone as the chlorophyll a extraction agent. The hot ethanol method is used widely in the world now but the cool acetone method is normally used in China. The results show that the hot ethanol method has more advantages than the cool acetone method, which are faster and easier to handle, more complete extraction and lower toxic. There is significant statistical difference between these two methods. By using the formula Chla ethanol =1.261 Chla acetone -3.5, the cool acetone method data can be transfered to the hot ethanol method data. |
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| Keywords: | Phytoplanktonic Chlorophyll a Cool acetone mehtod Hot ethanol method |
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