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浅水湖泊中碱性磷酸酶活性及其动力学参数的分层现象
引用本文:周易勇,李建秋,付永清,陈旭东,张玉敏.浅水湖泊中碱性磷酸酶活性及其动力学参数的分层现象[J].湖泊科学,2000,12(1):53-58.
作者姓名:周易勇  李建秋  付永清  陈旭东  张玉敏
作者单位:1. 中国科学院水生生物研究所,武汉430072
2. 黑龙江省桦南县环境保护局
基金项目:国家自然科学基金!(39670149、39170165),淡水生态与生物技术国家重点实验室基金,中国科学院东湖湖泊生态系统试
摘    要:1995年5月东湖与严西湖各采样点间歇水、上覆水和表层水中碱性磷酸酶的动力学参数(Vmax和Km值)明显不同(P〈0.05),与表层水相比,东湖Ⅰ站上覆水中酶的Vmax明显较高,严西湖各站上覆水中酶的Vmax明显较低,而在东湖Ⅱ站,上覆水中酶的Vmax和Km值明显较低,故仍有较高的催化效率。这一现象似与酶活性的组成有关。总之,富营养的东湖各站诸层次磷的酶促转换效率自上而下呈明显的递增趋势,严西湖则

关 键 词:分层  碱性磷酸酶  动力学  浅水湖泊  分层现象
收稿时间:1998/9/28 0:00:00
修稿时间:1998-09-28

Stratification of Activities and Kinetics of Alkaline Phosphatase in A Shallow Chinese Freshwater Lake (Donghu Lake, Wuhan)
ZHOU Yiyong,LI Jianqiu,FU Yongqing,CHEN Xudong and ZHANG Yumin.Stratification of Activities and Kinetics of Alkaline Phosphatase in A Shallow Chinese Freshwater Lake (Donghu Lake, Wuhan)[J].Journal of Lake Science,2000,12(1):53-58.
Authors:ZHOU Yiyong  LI Jianqiu  FU Yongqing  CHEN Xudong and ZHANG Yumin
Institution:ZHOU Yiyong ,LI Jianqiu ,FU Yongqing ,CHEN Xudong ,ZHANG Yumin ;(InStitute of Hydrobiology,Chinese Academy of Sciences.Wuhan 430072. P.R.China)
Abstract:In May 1995, the kinetic parameters (Vmax and Km) of alkaline phosphatase in interstitial water, overlying water and surface water were differed significantly in Donghu Lake (Wuhan) and Yanxihu Lake(P<0.05).At Station I and HI of Donghu Lake, Vmax values of overlying water were much higher than those of surface water, while in Yanxihu Lake Vmax values of overlying water were much lower.At Station II in Donghu Lake, both Vmax and Km values were quite lower in overlying water, exhibiting a higher catalytic efficiency.This phenomenon might relate with the compositions of enzyme activities.In brief, a pronounced downward gradient of enzyme catalytic efficiency was observed in Donghu Lake, which was not recorded in Yanxihu Lake.Consequently, the enzymatic hydrolyzable P may contribute to the internal loading of the eutrophic lake, and stratification of kinetics and compositions of phosphatase in shallow lake is theoretically noticeable.
Keywords:Stratification  alkaline phsophatase  kinetics  shallow lake
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