| 三峡库区浮游生物群落结构与DNA多态性比较分析 |
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| 引用本文： | 邓文娜,余育和,冯伟松,颜庆云,宋晓红,刘志新.三峡库区浮游生物群落结构与DNA多态性比较分析[J].湖泊科学,2007,19(3):333-339. |
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| 作者姓名： | 邓文娜 余育和 冯伟松 颜庆云 宋晓红 刘志新 |
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| 作者单位： | 1. 中国科学院水生生物研究所,武汉,430072;中国科学院研究生院,北京,100039
2. 中国科学院水生生物研究所,武汉,430072 |
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| 基金项目： | 国家自然科学基金
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国家重点基础研究发展计划(973计划) |
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| 摘 要： | 以三峡库区所设A、B、C、D、E、F和G等7个站点为研究区域,利用RAPD技术就其浮游生物群落遗传多样性与物种多样性的关系进行了探索性研究.共鉴定出浮游生物41种,其中藻类4种,原生动物10种,轮虫类19种,枝角类4种,桡足类4种.物种组成相似性最高的是B站和C站,最低的是A站和F站.而站点间浮游生物群落遗传距离分析表明:相距最近的是C站与D站,最远的为D站与F站.树状聚类图也显示:各站点物种组成聚为一类;而DNA多态性RAPD最先聚在一起的是B与F,它们的对应枝由A、C、D、E构成,G单独成为一枝.研究表明,所发现的物种组成与所揭示的遗传多样性并未完全吻合.这或许是物种信息的缺失(如藻类与细菌),或许是RAPD技术的缺陷,或许两者所致.因此,本研究积累了三峡库区浮游生物群落遗传多样性的一些背景资料,而要阐释浮游生物群落遗传多样性与物种多样性之间的相互关系则有赖于应用灵敏的、重复性好的,且能分别分析原核和真核生物的DNA分子标记技术.
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| 关 键 词： | 三峡库区 浮游生物 RAPD 遗传多样性 物种多样性 三峡库区 浮游生物 群落结构 多态性 比较 分析表 region Reservoir community plankton DNA polymorphism species composition 标记技术 分子 真核生物 原核 应用 相互关系 阐释 背景资料 |
| 收稿时间： | 2006-09-30 |
| 修稿时间： | 2006-09-302007-02-25 |
Comparative analysis between species composition and DNA polymorphism of plankton community in the Three-Gorges Reservoir region |
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| DENG Wenn,YU Yuhe,FENG Weisong,YAN Qingyun,SONG Xiaohong and LIU Zhixin.Comparative analysis between species composition and DNA polymorphism of plankton community in the Three-Gorges Reservoir region[J].Journal of Lake Science,2007,19(3):333-339. |
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| Authors: | DENG Wenn YU Yuhe FENG Weisong YAN Qingyun SONG Xiaohong and LIU Zhixin |
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| Institution: | 1 . Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China; 2. Graduate School of the Chinese Academy of Sciences, Beifing 100039, P. R. China |
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| Abstract: | Recently,molecular ecology has been appearing to increasing attention,many kinds of molecular meth- ods were applied in this field.In addition,various experts have been interesting in the Three-Gorges Reservoir re- gion (TGRR) and even the whole Yangtze drainage basin after the beginning of dam engineering.However,few studies were focused on the molecular ecology of TGRR.In order to explore the relationship between genetic diver- sity and species diversity,the whole DNA of plankton communities from seven samples locations in the TGRR was analyzed by random amplified polymorphic DNA (RAPD),and species of which were identified as well.The 7 lo- cations can be clustered into 3 groups by genetic diversity.Station A,C,D and E cluster as one group,station B and F cluster as another group,and station G is different from all of others.While these stations still as a whole af- ter analyzing by RAPDistancel.04.Also,41 species were found in all,which contains 4 algae,10 protozoa,19 rotifer,4 cladocera and copepoda respectively.Meanwhile,the nearest genetic distance is only 0.2586 which oc- curred between station D and C,and the opposite one lies between D and F which is 0.4758.But species diversity analysis displays that station B and C have the nearest distance;correspondingly,A and F have the furthest one.In other words,the resuh of genetic diversity is not consistent with that of species diversity.Therefore,more informa- tion has been obtained from genetic diversity in river,which applied new information for establishing the relation- ship between genetic diversity and species diversity of plankton.And more molecular biological methods should be applied,such as that more sensitive as well as good replied approaches could analyze pronucleus and eukaryon re- spectively. |
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| Keywords: | Three-Gorges Reservoir Region(TGRR) plankton RAPD genetic diversity species diversity |
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