Transformation of Tobacco with ScMTII Gene‐Enhanced Cadmium and Zinc Accumulation |
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| Authors: | Hatice Daghan Mehmet Arslan Veli Uygur Nurcan Koleli |
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| Institution: | 1. Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Mustafa Kemal University, Hatay, Turkey;2. Department of Field Crops, Faculty of Agriculture, Mustafa Kemal University, Hatay, Turkey;3. Department of Environmental Engineering, Faculty of Engineering, Mersin University, Mersin, Turkey |
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| Abstract: | Genetic transformation is gaining importance for developing plant types suitable to metal accumulate and/or hyperaccumulate. In this study, the transgenic tobacco plant which transferred the ScMTII gene from Saccharomyces cerevisiae to wild type tobacco cultivar Petite Havana (SR1) was grown on soils with low and high cadmium (Cd) and zinc (Zn) concentrations in a growth chamber for 6 weeks and compared to wild type tobacco for Cd and Zn accumulation. Cadmium and Zn accumulations in the transgenic and wild type tobacco plants were increased with the increasing Cd and Zn concentrations. Unlike Zn, the transgenic plant accumulated significantly higher amount of Cd compared to the wild type control plants. Shoot Cd concentrations of transgenic tobacco in higher Cd dosages reached the above the hyperaccumulation threshold value of 100 mg Cd kg?1 in the dry weight (DW). Transgenic tobacco accumulated 354, 400, 372, and 457 mg Cd kg?1 DW, for 10, 20, 40, and 80 mg Cd kg?1 soil treatments, respectively. These values are 3.5–4.5‐fold higher than that of Cd hyperaccumulation threshold value. With 10 mg kg?1 Cd treatment, the bioconcentration factor (BCF) of transgenic tobacco plants for Cd reached up to 35 in which the threshold value for BCF should be at least 10. Our results showed that the transgenic tobacco may be used as a good Cd hyperaccumulator plant and for phytoextraction of Cd contaminated soils, but not for Zn. |
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| Keywords: | Hyperaccumulator plant Metallothionein Nicotiana tabacum Phytoextraction Transgenic tobacco |
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