The Sebei gasfield is the largest biogas accumulation found in China and many reservoirs and seal rocks superposed on a syndepositional anticline in Quaternary. The biogas charging and dissipating process and its distribution have been a research focus for many years. The authors suggest a diffusing and accumulating model for the biogas, as they find that the shallower the gas producer, the more methane in the biogas, and the lighter stable carbon isotope composition of methane. Based on the diffusing model, diffused biogas is quantitatively estimated for each potential sandy reservoir in the gasfield, and the gas charging quantity for the sandy reservoir is also calculated by the diffused gas quantity plus gas reserve in-place. A ratio of diffusing quantity to charging quantity is postulated to describe biogas accumulating state in a sandy reservoir, if the ratio is less than 0.6, the reservoir forms a good gas-pool and high-production layer in the gasfield, which often occurs in the reservoirs deeper than 900 m; if the ratio is greater than 0.6, a few gas accumulated in the reservoir, which frequently exists in the reservoirs shallower than 900 m. Therefore, a biogas accumulation model is built up as lateral direct charging from gas source for the sands deeper than 900 m and indirect charging from lower gas-bearing sands by diffusion at depth shallower than 900 m. With this charging and diffusion quantitative model, the authors conducted re-evaluation on each wildcat in the central area of the Qaidam Basin, and found many commercial biogas layers.