^a Catchment Hydrology Program, Melbourne Water, P.O. Box 4342, Melbourne, Vic. 3001, Australia

^b Department of Agriculture and Forestry, University of Melbourne, Parkville, Vic. 3052, Australia

Research by Melbourne Water has established that streamflow is significantly influenced by forest age. It is hypothesised that this relationship, and the observed decay after patch cutting in Black Spur 1, is largely the result of variation in transpiration. To study the relationship between forest age and transpiration in detail, sap velocity was measured over two summers in four mountain ash plots using the heat-pulse method. The trees on these plots were 50, 90, 150 and 230 years old. On days when measurements were made, the mean sap velocity was not significantly different in the 50-, 90- 230-year-old stands, but was significantly smaller by 14% in the 150-year-old stand. Overstorey sapwood area gradually decreased with plot age, and was 57% lower in the 230-year-old plot than in the 50-year-old plot. When combined with the sap velocity measurements, these data indicated that over the six warmest months of the year, transpiration in the 50-year-old plot was 190 mm more than in the 230-year-old plot. These results support a hypothesis that differences in streamflow between 50-year-old and 230-year-old mountain ash forest can largely be accounted for by differences in transpiration. Further heat-pulse studies in young regrowth and in thinned and unthinned 1939 regrowth will be needed if the streamflow changes in Black Spur 1 and Black Spur 3 are to be fully explained.