Degradation and formation of refractory DOM by bacteria during simultaneous growth on labile substrates and persistent lake water constituents |
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| Authors: | Dr Annette Geller |
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| Institution: | (1) Present address: Limnological Institute, University of Constance, P.O. Box 5560, D-7750 Konstanz, FRG |
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| Abstract: | Changes in molecular size distribution associated with degradation of refractory DOM (macromolecules, apparent mol wt. ≥1500)
by 3 strains of bacteria were investigated by Sephadex G-15 gel permeation chromatography and DOC analysis of the eluates.
Macromolecules and bacteria were isolated from the same lake water sample, one taken in summer and one in winter. The decompositional
changes of the DOM fraction were compared with respect to substance- and bacterial species-specific differences, and with
respect to the action of photolysis and co-substrate supplementation. The metabolite patterns resulting from the simultaneous
growth of the bacteria on the persistent DOM fraction and the labile co-substrate, glutamic acid were analyzed. The macromolecules
differed in accessible components, and the bacteria degraded most effectively the DOM fraction of the parent lake water sample.
Photolysis was the prerequisite for the reduction of the inaccessible bulk of the macromolecules. Glutamic acid enhanced the
degradation of the macromolecules. The enhancement effect was impaired by the build up of waste products which balanced the
losses of the DOM fraction. Three formation modes of refractory metabolites could be distinguished: formation of intensely
UV absorbing small sized products which were poor in DOC during degredation of (1) the macromolecules of winter, and (2) of
glutamic acid, and formation of (3) apparent high molecular weight substances from glutamic acid in cultures containing the
macromolecules of summer which probably results from a stable linkage between the small sized metabolites of the amino acid
and the DOM fraction.
The research was supported by the Deutsche Forschungsgemeinschaft. |
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