In order to study the influence of pile spacing on the seismic response of piled raft in soft clay, a series of shaking table tests were conducted by using a geotechnical centrifuge. The dynamic behavior of acceleration, displacement and internal forces was examined. The test results indicate that the seismic acceleration responses of models are generally greater than the surrounding soil surface in the period ranges of 2–10 seconds. Foundation instant settlements for 4×4 and 3×3 piled raft (with pile spacing equal to 4 and 6 times pile diameter) are somewhat close to each other at the end of the earthquake, but reconsolidation settlements are greater for 3×3 piled raft. The seismic acceleration of superstructure, the uneven settlement of the foundation and the maximum bending moment of pile are relatively lower for 3×3 piled raft. Successive earthquakes lead to the softening behavior of soft clay, which causes a reduction of the pile bearing capacity and thus loads are transferred from the pile group to the raft. For the case of a 3×3 piled raft, there is relatively smaller change of the load sharing ratio of the pile group and raft after the earthquake and the distribution of maximum bending moments at the pile head is more uniform.