PAH-degrading microbial consortium and its pyrene-degrading plasmids from mangrove sediment samples in Huian, China |
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| Authors: | Lin Yi Cai Li-Xi |
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| Institution: | Department of Bioengineering and Biotechnology, HuaQiao University, Quanzhou 362021, China. lyhxm@hqu.edu.cn |
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| Abstract: | PAH-degrading microbial consortium and its pyrene-degrading plasmids were enriched from the sediment samples of Huian mangroves. The consortium YL showed degrading abilities of 92.1%, 87.6%, 92.3%, and 95.8% for pyrene, fluoranthene, phenanthrene, and fluoene at 50 mg l(-1) after 21 days incubation, respectively. The dynamics of pH changes in the cultures was consistent with that of PAH concentration change. Bacillus cereus Py5 and Bacillus megaterium Py6 were isolated from the consortium and observed consuming 65.8% and 33.7% of pyrene (50 mg l(-1)) within three weeks, respectively. The enriched Escherichia coli DH5alpha cells containing the plasmids of YL were demonstrated to degrade 85.7% of the original pyrene concentration at the 21st day. |
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| Keywords: | Polycyclic aromatic hydrocarbons (PAHs) Mangrove sediment Microbial consortium Bacillus megaterium Pyrene-degrading plasmids |
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