|
|||||
|
|
Mutation analysis of ras gene in the liver of European eel (Anguilla anguilla L.) exposed to benzo[a]pyrene |
|
| Authors: | Nogueira Patrícia R Lourenço Joana Mendo Sónia Rotchell Jeanette M |
| Institution: | aDepartamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal bDepartment of Biology and Environmental Science, University of Sussex, Falmer, Brighton BN1 9QJ, UK |
| Abstract: | Ras is regarded as one of the most important genes involved in carcinogenesis. Such genes have been characterised in several fish species and the presence of ras mutations have already been described in fish populations from hydrocarbon contaminated areas and following experimental exposure to specific contaminants. The aims of this study were to evaluate the DNA integrity by comet assay, to isolate the normal ras gene of Anguilla anguilla and analyse for the presence of ras gene mutations or changes in gene expression levels induced after one month of benzoa]pyrene (BaP) experimental exposure. The A. anguilla ras cDNA isolated revealed a 189 amino acid protein and alignment with other vertebrate ras proteins revealed conservation of functionally important regions. Following experimental exposure to BaP, an increase in DNA damage was found by comet assay. However, no point mutations or changes in ras gene expression levels were detected when compared to control samples. In contrast to the majority of fish ras gene sequences, a high degree of polymorphic variation was detected in the A. anguilla ras gene. |
| Keywords: | Ras Mutation A anguilla |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |