| 应用CTPI后的延迟扫描评价BBB破坏的价值研究 |
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| 引用本文： | 杨春,徐凯.应用CTPI后的延迟扫描评价BBB破坏的价值研究[J].CT理论与应用研究,2007,16(3):60-64. |
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| 作者姓名： | 杨春 徐凯 |
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| 作者单位： | 徐州医学院附属医院,影像科,江苏,徐州221002 |
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| 摘 要： | 目的:研究多层螺旋CT灌注成像(CT perfusion imaging,CTPI)后的延迟扫描成像在大鼠大脑中动脉栓塞及再灌注过程中的变化过程,结合组织病理学(光镜、电镜)结果,探讨CTPI后的延迟扫描成像在评价急性脑缺血血脑屏障(blood-brain barrier,BBB)破坏中的应用价值.材料和方法:采用线栓法制备大鼠可逆性右侧大脑中动脉栓塞模型.将25只SD大鼠随机分为5组:假手术组,缺血2h再灌注2h组(I2hR2h组),缺血2h再灌注24h组(I2hR24h组),缺血6h再灌注2h组(I6hR2h组),缺血6h再灌注24h组(I6hR24h组),每组各5只.各组分别于各自时间点行常规平扫、CTPI,并于延迟2min后行CT平扫(延迟扫描),扫描完成后立即处死行组织病理学(光镜、电镜)检查.结果:A组延迟扫描两侧大脑半球密度无明显变化,B组、C组、D组、E组缺血再灌注后右侧大脑半球延迟扫描的CT值均较延迟扫描前有升高,其中E组延迟扫描后右侧纹状体出现斑片状强化,与其他各组比较其CT值变化有统计学意义.组织病理学(光镜、电镜)检查结果也显示E组病理损伤最为严重,光镜下可见胞浆呈粉红色并可见核溶解改变,电镜下可见毛细血管基底膜断裂,管腔变形狭窄.结论:CTPI后的延迟扫描可以用来评价急性脑缺血再灌注后血脑屏障的破坏情况.
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| 关 键 词： | 计算机体层摄影术 脑缺血 血脑屏障 大鼠 |
Investigation of blood-brain barrier damage using delayed scan after CT perfusion imaging |
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| YANG Chun,XU Kai.Investigation of blood-brain barrier damage using delayed scan after CT perfusion imaging[J].Computerized Tomography Theory and Applications,2007,16(3):60-64. |
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| Authors: | YANG Chun XU Kai |
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| Abstract: | Objective.To study the changes of delayed scan after CT perfusion imaging in the right middle cerebral artery occlusion of rats,combining with histologic outcomes(light and electron microscopy).To investigation of blood-brain barrier damage after acute ischemic stroke.Methods.All 25 rats were divided into 5 groups.And the MCAO(middle cerebral artery occlusion) model were set up.Every groups performed routine scan,CT perfusion imaging(CTPI),and delayed scan in turn.All rats were killed immediately after scan and dealt with light and electron microscopy.Results.In group A,no abnormal outcomes were found in delayed scan.In group E,enhancement in the ipsilateral cortex in delayed scan were seen.And in light and electron microscopy most neurons were markedly shrunken,some of them had pyknotic nuclei and eosinophilic cytoplasm.Conclusion.Delayed scan can evaluate blood-brain barrier damage of acute ischemic stroke. |
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| Keywords: | computed tomography cerebral ischemia blood-brain barrier rat |
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