| 双源CT诊断兔急性肺栓塞实验研究 |
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| 引用本文： | 廖健,张晓琴,王敏,张凯,董宇清,云峰.双源CT诊断兔急性肺栓塞实验研究[J].CT理论与应用研究,2015,24(6):769-775. |
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| 作者姓名： | 廖健 张晓琴 王敏 张凯 董宇清 云峰 |
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| 作者单位： | 1. 内蒙古医科大学研究生学院, 呼和浩特 010110; |
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| 基金项目： | 内蒙古自治区自然科学基金 |
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| 摘 要： | 目的:应用双源CT探讨新西兰兔耳缘静脉注入明胶海绵制造急性肺动脉栓塞(PE)模型的可行性。方法:新西兰兔24只随机分为实验组(n=22只,按栓塞后检查时间分为2h、1d、3d和7d组)与对照组(n=2只)。实验组22只采用经耳缘静脉快速注射明胶海绵栓子制成急性PE模型,栓塞前后均行CTPA及肺灌注检查,检查结束后全部处死。对照组2只新西兰兔经耳缘静脉注入等渗盐水后立即处死。全部行病理检查。结果:22只实验组新西兰兔制模成功20只,其中有2只分别因为栓塞过量和麻醉过量而未取得数据,模型制备成功率为90%。以肺叶为单位,在CT图像上分析100个肺叶影像表现,可见栓塞后2h组对应的肺叶纹理稀疏12叶;1d及3d组对应肺叶呈磨玻璃改变22叶;7d组对应肺叶实变3叶。DEPI均呈现低灌注。病理检查发现:2h组相应肺组织呈鲜红色,未见结构破坏及肺泡渗出;1d组对应肺组织呈水肿、淤血、出血及炎细胞浸润改变;3d组部分肺组织轻度梗死,对应肺组织呈深红色,肺泡间隔增宽,大量炎细胞浸润;7d组肺泡腔被渗出物填充,对应肺组织呈暗红色,实变坏死。实验组共发现3只新西兰兔肺动脉管腔内含明胶海绵。结论:经新西兰兔耳缘静脉注射明胶海绵制作急性肺栓塞动物模型操作简单,成本低廉,成功率较高,是肺栓塞影像学研究较容易制作的实验模型。
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| 关 键 词： | 肺动脉栓塞 CT肺动脉成像 肺灌注 动物模型 |
| 收稿时间： | 2015-09-29 |
Dual-source CT in the Diagnosis of Acute Pulmonary Embolism Rabbit Experimental Study |
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| Institution: | 1. Inner Mongolia Medical University Graduate School, Huhhot 010110, China;2. Department of Medical Imaging, Inner Mongolia People's Hospital, Huhhot 010017, China |
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| Abstract: | Study the feasibility of manufacturing the model of acute pulmonary embolism by injecting gelatin sponge from New Zealand rabbit ear margin vein by dual-source CT. Methods: 24 New Zealand rabbits divided into two groups: experimental group(n=22) and control group(n=2). In experimental group, we injected gelatin sponge into rabbit's ear margin vein, and divided them into 2houes, 1 day, 3 days, 7 days group. All proceed CTPA and lung perfusion examination before and after embolization. After the inspection, all rabbits put to death. Control group injected isotonic saline into rabbit's ear margin vein. After the inspection, all rabbits put to death. All groups put to pathological examination. Results: In the experimental group, 20 New Zealand rabbits molding success, 2 rabbits have not obtained data because of embolization excess and anesthesia excess, the success rate of 90%. Analysis on CT images of 100 lung lobes, In 2 hours group, there are 12 lung lobes texture sparse; in 1d and 3d groups there are 22 lung lobes in ground-glass attenuation; in 7d group there are 3 lung lobes consolidation. DEPI show low perfusion in experimental group. Pathological examination revealed: The lung tissue in 2 hours group was bright red, no structural damage and alveolar exudates; In 1 day group the lung tissue was edema, congestion, hemorrhage and inflammatory cell infiltration; The lung tissue in 3 day group was deep red, the alveolar interval broaden, a large number of inflammatory cells infiltration, some lung tissue necrosis; The lung tissue in 7 day group was dark red, lung tissue necrosis, the alveolar cavity is filled with exudates. The experimental group was found three New Zealand rabbit pulmonary artery lumen with gelatin sponge. Conclusions: Injection of gelatin sponge By the New Zealand rabbit ear margin vein production of acute pulmonary embolism animal model is simple operation, low cost, and high success rate. It is a experimental model which relatively easy to make in imaging studies of pulmonary embolism. |
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| Keywords: | PE CTPA DEPI animal models |
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