Dissolved free amino acids in coastal seawater using a modified fluorometric method |
| |
| Authors: | Kuninao Tada Mitsutoshi Tada Yoshiaki Maita |
| |
| Institution: | (1) Faculty of Agriculture, Kagawa University, Miki, 761-0701 Kagawa, Japan;(2) Faculty of Fisheries, Hokkaido University, Minato-cho 3-1-1, 041-0821 Hakodate, Japan |
| |
| Abstract: | The method of Parsonset al. (1984) for measuring dissolved free amino acids (DFAA) in coastal seawater was modified. We found considerable interference
in DFAA determination from ammonia dissolved in coastal seawater, although the interference of urea could be ignored. For
DFAA analysis for coastal seawater samples, ammonia determinations for the same sample are needed to correct DFAA values.
For coastal surface seawater samples from all over the Seto Inland Sea, Japan, values of DFAA ranged from undetectable to
1.87 μg-at N/l when corrected for ammonia, while uncorrected values ranged from undetectable to 2.61 μg-at N/l. DFAA, urea,
nitrate+nitrite, ammonia and DON concentrations in surface seawater collected in the Seto Inland Sea were analyzed simultaneously.
DFAA at four seasons constituted from 1.4 to 10.1% of DON, with a mean value of 6.5%. The concentration of urea was similar
to that of DFAA and often higher than that of ammonium, although generally lower than that of nitrate. |
| |
| Keywords: | DFAA fluorometric method the Seto Inland Sea DON urea |
| 本文献已被 SpringerLink 等数据库收录！ |
|
