| 鳗鲡(Anguilla japonica)性腺发育和饥饿胁迫下生物学指标及体内蛋白质与氨基酸含量变化 |
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| 引用本文： | 王婷,刘利平,陈桃英,张利娜,林静,王倩,帅滇.鳗鲡(Anguilla japonica)性腺发育和饥饿胁迫下生物学指标及体内蛋白质与氨基酸含量变化[J].海洋与湖沼,2015,46(6):1373-1379. |
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| 作者姓名： | 王婷 刘利平 陈桃英 张利娜 林静 王倩 帅滇 |
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| 作者单位： | 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306 |
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| 基金项目： | 上海市科学技术委员会项目, 13320502200 号, 08391910200 号; 上海市农委科技攻关项目, 沪农科攻字2013 第2-2 号; 上 海海洋大学科技发展专项基金项目, A2-0209-15-200005 号。 |
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| 摘 要： | 自然条件下, 鳗鲡(Anguilla japonica)自降河入海至性腺发育成熟需洄游数千公里、耗时半 年以上, 且整个过程中不摄食。本文以降海洄游、人工促熟和长期饥饿(20 个月)三种状态下的鳗鲡 为材料, 研究其在性腺发育和饥饿胁迫时的主要生物学指标(包括肥满度、肝体比、脏体比、性腺指 数、含肉率)以及肌肉、肝脏和性腺中的粗蛋白与氨基酸含量的变化情况, 以探讨鳗鲡性腺发育和饥 饿胁迫下的营养物质来源与变化。结果发现: 人工促熟鳗鲡的肝体比、脏体比和性腺指数显著高于 降海洄游鳗鲡(P<0.05), 而含肉率、肝脏和卵巢内的粗蛋白含量、氨基酸总量、必需氨基酸总量以及 非必需氨基酸总量均显著低于降海洄游鳗鲡的含量(P<0.05); 长期饥饿鳗鲡的肥满度、肝体比、含肉 率、肌肉(鲜样)、肝脏内的粗蛋白、氨基酸总量、必需氨基酸总量以及必需氨基酸占氨基酸总量的 比值显著低于降海洄游鳗鲡(P<0.05).结果表明, 鳗鲡性腺发育所需要的营养物质主要来源于肌肉, 其性腺的发育依赖自身营养物质在体内的流动和转化, 肌肉中的必需氨基酸和非必需氨基酸均被利 用, 转化成性腺发育所需要的物质。
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| 关 键 词： | 鳗鲡 性腺发育 饥饿胁迫 生物学指标 粗蛋白 氨基酸 |
| 收稿时间： | 2015/2/27 0:00:00 |
| 修稿时间： | 2015/5/24 0:00:00 |
VARIATIONS OF BIOLOGICAL INDICATORS AND PROTEIN AND AMINO ACID CONTENT IN ANGUILLA JAPONICA DURING GONAD DEVELOPMENT AND STARVATION STRESS |
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| WANG Ting,LIU Li-Ping,CHEN Tao-Ying,ZHANG Li-N,LIN Jing,WANG Qian and SHUAI Dian.VARIATIONS OF BIOLOGICAL INDICATORS AND PROTEIN AND AMINO ACID CONTENT IN ANGUILLA JAPONICA DURING GONAD DEVELOPMENT AND STARVATION STRESS[J].Oceanologia Et Limnologia Sinica,2015,46(6):1373-1379. |
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| Authors: | WANG Ting LIU Li-Ping CHEN Tao-Ying ZHANG Li-N LIN Jing WANG Qian and SHUAI Dian |
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| Institution: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China and Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China |
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| Abstract: | We investigated the changes of major biological indices (the condition factor, hepatosomatic index, viscerosomatic index, and gonadosomatic index), meat content ratio, and the contents of crude protein and amino acid in muscle, liver, and gonads among three groups of eel Anguilla japonica, i.e., artificially-induced maturation eels, long-term fasting eels (20 months), and wild broodstock eels. The results show that the hepatosomatic index, viscerosomatic index, and gonadosomatic index of induced maturation eels were significantly greater than that of wild broodstock eel (P<0.05), while the meat content, crude protein content, total amino acids, total essential amino acids and the total non-essential amino acids content in liver and ovary were significant smaller than those of wild broodstock eel (P<0.05). The condition factor, hepatosomatic index, meat content, crude protein, total amino acids, total essential amino acids, and total non-essential amino acids in muscle and liver of long fasting eels (20 months) were significantly smaller than those of the wild broodstock eels (P<0.05). The results imply that the nutrients required by A. japonica during gonad development were supplied from muscle, and both essential amino acids and non-essential amino acids were utilized to meet the nutrients demand from gonadal development. |
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| Keywords: | Anguilla japonica gonad development starvation stress biological indices crude protein amino acid |
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