| 南海海域红斑后海螯虾(Metanephrops thomsoni)的分子识别与群体遗传分化 |
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| 引用本文： | 范艳波,王中铎,郭昱嵩,颜云榕,刘楚吾.南海海域红斑后海螯虾(Metanephrops thomsoni)的分子识别与群体遗传分化[J].海洋与湖沼,2013,44(1):220-225. |
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| 作者姓名： | 范艳波 王中铎 郭昱嵩 颜云榕 刘楚吾 |
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| 作者单位： | 1. 广东海洋大学水产学院 南海水产经济动物增养殖广东普通高校重点实验室 湛江 524025
2. 广东海洋大学水产学院 南海水产经济动物增养殖广东普通高校重点实验室 湛江 524025;广东海洋大学南海渔业资源监测与评估中心 湛江524088 |
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| 基金项目： | 国家自然科学基金资助项目, 31101904 号, 31201996 号; 广东省科技计划项目资助, 2010B020308004 号 |
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| 摘 要： | 利用16S rRNA基因序列分子识别红斑后海螯虾,然后对海南附近海域(18°30′-19°00′N,111 °30′-112°30′ E)、北部湾口海域(15°41′ N,110°40′ E)和南沙群岛海域(5°20′-5°29′N,110°09′-111°26′E)三个地理群体进行ITS1的扩增测序.16S rRNA基因序列的结果表明所取样本准确;三个地理群体分别得到616-623bp、619bp和614bp的ITS1全长序列,A、G、T、C含量平均分别为22.3％、29.4％、17.8％和30.6％;其中562个保守位点,39个多态位点.ML和NJ聚类分析显示三个地理群体共聚为三个大支,其中海南群体有群体分化.表明红斑后海螯虾三个地理群体分化极其显著,且海南群体具有相对高的遗传多样性.
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| 关 键 词： | 红斑后海螯虾 16S rRNA ITS1 遗传多样性 |
| 收稿时间： | 2012/2/13 0:00:00 |
| 修稿时间： | 5/6/2012 12:00:00 AM |
MOLECULAR RECOGNITION AND POPULATION GENETIC DIFFERENTIATION OF METANEPHROPS THOMSONI IN SOUTH CHINA SEA |
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| FAN Yan-Bo,WANG Zhong-Duo,GUO Yu-Song,YAN Yun-Rong and LIU Chu-Wu.MOLECULAR RECOGNITION AND POPULATION GENETIC DIFFERENTIATION OF METANEPHROPS THOMSONI IN SOUTH CHINA SEA[J].Oceanologia Et Limnologia Sinica,2013,44(1):220-225. |
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| Authors: | FAN Yan-Bo WANG Zhong-Duo GUO Yu-Song YAN Yun-Rong and LIU Chu-Wu |
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| Institution: | Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University;Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University;Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University;Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University; Center of South China Sea Fisheries Resources
Monitoring and Assessment, Guangdong Ocean University;Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University |
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| Abstract: | |
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| Keywords: | Metanephrops thomsoni 16S rRNA ITS1 Genetic diversity |
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