| 斑点叉尾(Ictalunes punctatus)源鲁氏耶尔森氏菌的分离鉴定及系统发育分析 |
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| 引用本文： | 范方玲,汪开毓,耿 毅,黄小丽,陈德芳.斑点叉尾(Ictalunes punctatus)源鲁氏耶尔森氏菌的分离鉴定及系统发育分析[J].海洋与湖沼,2010,41(6):862-868. |
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| 作者姓名： | 范方玲 汪开毓 耿 毅 黄小丽 陈德芳 |
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| 作者单位： | 四川农业大学鱼病研究中心,动物疫病与人类健康四川省重点实验室,雅安,625014 |
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| 基金项目： | 教育部“长江学者和创新团队发展计划”创新团队项目, IRT0848 号; 农业部动物疫病与防治项目, 2130108 号 |
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| 摘 要： | 从发病的斑点叉尾肝脏和肾脏均分离到一高致病性的菌株(FF003),感染该菌后斑点叉尾表现为体表多处点状出血,特别是在下颌、腹壁、体侧处,各内脏器官不同程度出血,尤其是腹内膜和鳔内膜斑块状出血。该菌为非发酵型需氧,革兰氏阴性杆菌,对除麦芽糖、甘露糖、果糖、海藻糖、D-甘露醇以外的多种糖类不利用产酸,氧化酶阴性,精氨酸双水解酶、鸟氨酸脱羧酶、赖氨酸脱羧酶阳性,MR阳性。在以该菌16S rDNA序列(GenBank登录号为FJ908709)和GenBank数据库内同源性较高的细菌16SrDNA序列构建的系统发育树中,分离菌FF003株与鲁氏耶尔森氏菌(Yersinia ruckeri)聚为一个分支,结合形态和生理生化特点将其鉴定为鲁氏耶尔森氏菌。药敏实验结果表明氟哌酸、环丙沙星、强力霉素、氯霉素对其高度敏感,头孢拉定、克拉霉素、磺胺甲基异唑、庆大霉素、麦迪霉素等药物不敏感。
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| 关 键 词： | 斑点叉尾 鲁氏耶尔森氏菌 16S rDNA 系统发育 药敏试验 |
| 收稿时间： | 2009/7/25 0:00:00 |
ISOLATION, IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF YERSINIA RUCKERI IN CHANNEL CATFISH ICTALUNES PUNCTATUS |
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| FAN Fang-Ling,WANG Kai-Yu,GENG Yi,HUANG Xiao-Li and CHEN De-Fang.ISOLATION, IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF YERSINIA RUCKERI IN CHANNEL CATFISH ICTALUNES PUNCTATUS[J].Oceanologia Et Limnologia Sinica,2010,41(6):862-868. |
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| Authors: | FAN Fang-Ling WANG Kai-Yu GENG Yi HUANG Xiao-Li and CHEN De-Fang |
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| Institution: | Fish Disease Research Center of Sichuan Agriculturical University; Key Laboratory of Sichuan Provincial Animal Disease and Human Health;Fish Disease Research Center of Sichuan Agriculturical University; Key Laboratory of Sichuan Provincial Animal Disease and Human Health;Fish Disease Research Center of Sichuan Agriculturical University; Key Laboratory of Sichuan Provincial Animal Disease and Human Health;Fish Disease Research Center of Sichuan Agriculturical University; Key Laboratory of Sichuan Provincial Animal Disease and Human Health;Fish Disease Research Center of Sichuan Agriculturical University; Key Laboratory of Sichuan Provincial Animal Disease and Human Health |
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| Abstract: | A bacterial strain named FF003 was isolated from the liver and kindey of the diseased channel catfish Ictalunes punctatus. FF003 had apparent pathogenicity to channel catfish. Symptoms in FF003-infected fish included punctate haemorrhaging on the body surface, especially on the submaxilla, abdominal wall and the side of the body. All visceral organs had different degree of hemorrhages; in particular peritoneum and the endomembrane of swim bladder showed punctate hemorrhage. The isolated strain was an aerobic, non-fermentative bacterium. FF003 cells were rod-shaped, Gram negative, Methyl red positive, oxidase negative, and arginine dihydrilase, rnithine decarboxylase and lysine decarboxylase positive. Many carbohydrates could not be utilized by FF003 for acid production, expect maltose, mannose, fructose, trehalose, and D- mannitol. A phylogenetic tree was constructed by comparing with the 16S rDNA sequence of the strain FF003 (GenBank accession number FJ908709) and other relative bacteria species in the GenBank. In the phylogenetic tree FF003 and Yersinia ruckeri constituted a branch. According to morphological, physiological and biochemical characteristics and phylogentic analysis, strain FF003 was identified as Y. ruckeri. Antibiotic sensitivity testing showed that FF003 was very sensitive to norfloxacin, ciprofloxacin, doxycycline and chloramphenicol, but not sensitive to cefradin, clarithromycin, sulfamethoxazole, gentamicin and midecamycin. |
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| Keywords: | Channel catfish Ictalunes punctatus Yersinia ruckeri 16S rDNA Phylogenetic analysis Antibiotic sensitivity testing |
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