| 条斑紫菜( Porphyra yezoensis) 耐高温品系的筛选及特性分析 |
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| 引用本文： | 王华芝,严兴洪,李 琳.条斑紫菜( Porphyra yezoensis) 耐高温品系的筛选及特性分析[J].海洋与湖沼,2012,43(2):363-369. |
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| 作者姓名： | 王华芝 严兴洪 李 琳 |
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| 作者单位： | 上海海洋大学水产与生命学院,上海,201306 |
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| 基金项目： | 国家高科技研究发展计划(863 计划) 资助项目, 2006AA10A413 号; 国家自然科学基金资助项目, 31072208 号; 农业部公益性专项, 200903030-C 号; 上海市科委重点科技攻关项目, 10391901100 号; 上海市优秀学科带头人项目, 07XD14028 号; 上海市教委重点学科建设项目, J50701 号 |
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| 摘 要： | 采用60Co-γ射线诱变和高温胁迫处理条斑紫菜野生品系(WT),进行了耐高温品系的选育研究。结果表明,在18℃和22℃组,所选育的耐高温品系(TM-18)和WT品系的壳孢子存活率无显著差异,但在24℃和25℃组,TM-18与WT相比,壳孢子存活率分别提高了274%和296%,畸形苗率分别下降了286%和161%,假根发生率分别提高了151%和429%。将18℃培养50d的苗再分别在18℃、22℃和24℃下再培养30d,TM-18苗的绝对生长率分别为WT苗的3.82、3.16和4.95倍,特定生长率为WT苗的1.53、1.49、2.10倍。在24℃下培养15d或25℃下培养10d,WT苗停止生长,出现了卷曲和严重腐烂,而TM-18苗仍然生长良好,不腐烂。另外,TM-18的三种主要光合色素(Chl.a、PE和PC)含量明显比WT高。上述结果说明,TM-18是一个颜色好、生长快和耐高温的优良品系。
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| 关 键 词： | 条斑紫菜 叶状体 壳孢子 耐高温 光合色素 |
| 收稿时间： | 2011/1/21 0:00:00 |
| 修稿时间： | 2011/3/28 0:00:00 |
SELECTION AND CHARACTERIZATION OF A HIGH-TEMPERATURE RESISTANT STRAIN OF PORPHYRA YEZOENSIS UEDA (BANGIALES, RHODOPHYTA) |
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| WANG Hua-Zhi,YAN Xing-Hong and LI Lin.SELECTION AND CHARACTERIZATION OF A HIGH-TEMPERATURE RESISTANT STRAIN OF PORPHYRA YEZOENSIS UEDA (BANGIALES, RHODOPHYTA)[J].Oceanologia Et Limnologia Sinica,2012,43(2):363-369. |
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| Authors: | WANG Hua-Zhi YAN Xing-Hong and LI Lin |
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| Institution: | College of Fisheries and Life Science , Shanghai Ocean University;College of Fisheries and Life Science , Shanghai Ocean University;College of Fisheries and Life Science , Shanghai Ocean University |
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| Abstract: | A high-temperature-tolerant strain(TM-18) of Porphyra yezoensis Ueda was isolated from the wild type(WT) using by 60 Co-γ-radiation and high-temperature cultivation.At 18 and 22℃,there was no significant difference on the survival rates between the WT and TM-18 conchospores.However,at 24 and 25℃,the survival rates and the rhizoid germination rates of conchospore-germlings of TM-18 were 274% and 296%,151% and 429% higher than those of the WT,respectively.Furthermore,the occurrence rates of abnormal blades in TM-18 was reduced by 286% and 161% compared with the WT.When the 50-day-old conchospore-germlings initially cultured at 18℃ were cultured at 18,22 and 24℃ for another 30 days,the specific growth rates of the TM-18 blades were 3.82,3.16 and 4.95 times higher than the WT,respectively,and the absolute growth rates of the TM-18 blades were 1.53,1.49 and 2.10 higher than the WT,respectively.The WT blades stopped growing and then severely decayed after being cultured for 15 days at 24℃ or 10 days at 25℃.In comparison,the TM-18 blades maintained normal growth.Finally,the contents of the three main photosynthetic pigments(Chl.a,PE and PC) in the TM-18 blades were significantly higher than those of the WT blades.This study showed that the TM-18 strain was superior to WT strain in growth,high-temperature tolerance,and pigment productions. |
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| Keywords: | Porphyra yezoensis Gametophytic blade Conchospore High-temperature-resistant Photosyn-thetic pigment |
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