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Cd2+与Zn2+的联合效应对马氏珠母贝(Pinctada martensii)受精率的影响
引用本文:邓云翠,王 辉,李艳红,朱晓闻,罗明明,王亚男.Cd2+与Zn2+的联合效应对马氏珠母贝(Pinctada martensii)受精率的影响[J].海洋与湖沼,2012,43(2):323-328.
作者姓名:邓云翠  王 辉  李艳红  朱晓闻  罗明明  王亚男
作者单位:广东海洋大学水产学院,湛江,524025
基金项目:广东省教育厅工程中心建设项目, GCZX-A0909 号; 广东省科技厅科技计划重大专项, 2010B020201014 号; 科技部国家星火计划项目, 2011GA780001 号
摘    要:采用中心组合设计和响应曲面法在实验室条件下研究了Cd2+与Zn2+对马氏珠母贝受精率的影响。Cd2+、Zn2+的浓度范围分别为0.030—20mg/L、0.100—20mg/L。结果表明:Cd2+与Zn2+两因子对马氏珠母贝受精率的一次效应极显著(P<0.01);Cd2+与Zn2+两因子一次互作效应极显著(P<0.01)。经响应曲面法分析,随着Cd2+与Zn2+浓度的增加受精率呈下降趋势,当Zn2+浓度在最低浓度(0.100mg/L)时,受精率随着Cd2+浓度的上升而下降;当Cd2+浓度在最低浓度(0.030mg/L)时,受精率随Zn2+浓度的上升而先下降后升高。本实验建立了马氏珠母贝受精率与Cd2+、Zn2+间关系的模型方程(R2=0.996,Adj.R2=0.987,Pred.R2=0.887,P<0.01)并可用于预测Cd2+、Zn2+对马氏珠母贝受精率的影响。

关 键 词:马氏珠母贝  受精率  Cd2+  Zn2+
收稿时间:2011/4/10 0:00:00
修稿时间:2011/6/23 0:00:00

COMBINED EFFECTS OF Cd2+ AND Zn2+ ON THE FERTILIZATION RATE IN PINCTADA MARTENSII (DUNKER)
DENG Yun-Cui,WANG Hui,LI Yan-Hong,ZHU Xiao-Wen,LUO Ming-Ming and WANG Ya-Nan.COMBINED EFFECTS OF Cd2+ AND Zn2+ ON THE FERTILIZATION RATE IN PINCTADA MARTENSII (DUNKER)[J].Oceanologia Et Limnologia Sinica,2012,43(2):323-328.
Authors:DENG Yun-Cui  WANG Hui  LI Yan-Hong  ZHU Xiao-Wen  LUO Ming-Ming and WANG Ya-Nan
Institution:Fisheries College of Guangdong Ocean University;Fisheries College of Guangdong Ocean University;Fisheries College of Guangdong Ocean University;Fisheries College of Guangdong Ocean University;Fisheries College of Guangdong Ocean University;Fisheries College of Guangdong Ocean University
Abstract:Using the central composite face-centered design(CCF) and response surface methodology,the combined effects of Cd2+(0.030—20.000mg/L) and Zn2+(0.100—20.000mg/L) on the fertilization rate in Pinctada martensii were studied under laboratory conditions.The results showed that the effects of two factors Cd2+ and Zn2+ on the fertilization rate in P.martensii were highly significant(P<0.01),and effect of Cd2+ was more significant than that of Zn2+.The interaction between the two factors was also highly significant(P<0.01).Based on the response surface plots,it was found that with the increased concentration of Cd2+ and Zn2+,the fertilization rate was decreased.When Zn2+ concentration was at its lowest level(0.100mg/L),the fertilization rate decreased with the increased Cd2+ concentration.When Cd2+ concentration was at its lowest level(0.030mg/L),the fertilization rate was first decreased and then increased as the concentration of Zn2+ increased.The model equation of the fertilization rate towards Cd2+ and Zn2+ was established,with R 2 = 0.996,Adj.R 2 = 0.987 and Pred.R 2 = 0.887(P<0.01).The model equation could be practically used for forecasting the effects of Cd2+ and Zn2+ on the fertilization rate in P.martensii.
Keywords:Pinctada martensii (Dunker)    Fertilization rate    Cd2+    Zn2+
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