|
|||||
|
|
| 我国东南沿海厚壳贻贝(Mytilus coruscus)4 个群体线粒体16S rRNA 序列及遗传结构分析 | |
| 引用本文： | 叶莹莹,徐梅英,郭宝英,吴常文.我国东南沿海厚壳贻贝(Mytilus coruscus)4 个群体线粒体16S rRNA 序列及遗传结构分析[J].海洋与湖沼,2012,43(2):376-381. |
| 作者姓名： | 叶莹莹 徐梅英 郭宝英 吴常文 |
| 作者单位： | 1. 浙江海洋学院海洋科学学院国家海洋设施养殖工程技术研究中心,舟山,316004 2. 浙江海洋学院海洋科学学院国家海洋设施养殖工程技术研究中心，舟山316004／浙江大海洋科技有限公司，舟山316004 3. 浙江海洋学院海洋科学学院国家海洋设施养殖工程技术研究中心，舟山316004／中国水产科学研究院东海水产研究所农业部海洋与河口渔业资源及生态重点开放实验室，上海200090 |
| 基金项目： | 国家海洋公益性行业科研专项, 201005013 号; 国家国际科技合作项目, 2010DFA22770 号; 农业部海洋与河口渔业资源及生态重点开放实验室开放基金, 10-01 号 |
| 摘 要： | 采用16S rRNA基因测序技术,对我国东南沿海4个地理群体的厚壳贻贝遗传结构及遗传变异进行研究。通过对4个厚壳贻贝群体共83个个体的线粒体16S rRNA基因进行测序,获得1个长度为305bp的同源序列,共检测到150个多态位点,多态位点比例达49.18%。83个个体中共检测到28个单倍型,单倍型多样性指数(Hd)为0.810,核苷酸多样性指数(Pi)为0.09602,平均核苷酸差异数(K)达27.846。结果表明,我国东南沿海厚壳贻贝群体具有较高的遗传多样性水平。遗传结构检测结果表明,舟山群体、温州群体、宁德群体间的遗传距离小,遗传分化系数(Fst)为-0.0141—0.0059之间,群体内部无显著分化(P>0.05),而福州群体与其它群体间遗传距离较大,为0.215—0.217之间,遗传分化系数(Fst)也较大,为0.6217—0.6319之间,存在极显著的遗传分化(P<0.001)。 |
| 关 键 词： | 厚壳贻贝 16S rRNA 遗传多样性 |
| 收稿时间： | 2010/12/29 0:00:00 |
| 修稿时间： | 3/2/2011 12:00:00 AM |
GENETIC STRUCTURE AND SEQUENCE ANALYSIS OF FOUR POPULATIONS OF MYTILUS CORUSCUS IN THE COASTAL WATERS OF SOUTHEAST CHINA SEA USING 16S rRNA |
|
| YE Ying-Ying,XU Mei-Ying,GUO Bao-Ying and WU Chang-Wen.GENETIC STRUCTURE AND SEQUENCE ANALYSIS OF FOUR POPULATIONS OF MYTILUS CORUSCUS IN THE COASTAL WATERS OF SOUTHEAST CHINA SEA USING 16S rRNA[J].Oceanologia Et Limnologia Sinica,2012,43(2):376-381. | |
| Authors: | YE Ying-Ying XU Mei-Ying GUO Bao-Ying and WU Chang-Wen |
| Institution: | Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Zhejiang Dahaiyang Science and Technology Co., Ltd.;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisherie;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture |
| Abstract: | The sequence of 16S rRNA was used to investigate the genetic structure and genetic variation of four populations of Mytilus coruscus in coastal waters of southeast China Sea.Partial sequence of 305bp was obtained through sequencing the 16S rRNA gene of 83 individuals from four M.coruscus populations.150 polymorphic sites,28 haplotypes were detected out of 83 individuals from four populations.The haplotype diversity index(Hd),mean nucleotide diversity index(P i) and average number of nucleotide differences(K) reached 0.810,0.09602 and 27.846,respectively.Results above showed that M.coruscus in coastal waters of southeast China Sea keep plentiful genetic variation in its natural populations.The genetic structure test results show that the genetic distance among the populations of Zhoushan,Wenzhou,and Ningde were so low,their F st among 0.0141—0.0059,no significant divergence was detected(P>0.05).The genetic distance between the population of Fuzhou and other three populations were higher.F st among 0.6217—0.6319,significant divergence was detected(P<0.001). |
| Keywords: | Mytilus coruscus 16S rRNA Genetic diversity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 | |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 | |