| 扇贝多肽在体外对免疫细胞活性的影响及其抗紫外线的氧化损伤作用 |
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| 引用本文： | 刘晓萍,王玉贞,韩彦弢,于业军,王跃军,王春波.扇贝多肽在体外对免疫细胞活性的影响及其抗紫外线的氧化损伤作用[J].海洋与湖沼,2001,32(4):414-419. |
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| 作者姓名： | 刘晓萍 王玉贞 韩彦弢 于业军 王跃军 王春波 |
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| 作者单位： | 1. 青岛大学医学院
2. 中国水产科学研究院黄海水产研究所 |
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| 基金项目： | 国家自然科学基金资助项目39970 638号 |
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| 摘 要： | 采用噻唑盐（MTT）比色方法研究在紫外线辐射损伤条件下扇贝多肽对免疫细胞的保护作用，并探讨扇贝多肽对胸腺细胞和脾细胞活性的影响，结果表明，扇贝多肽具有抗紫外线氧化损伤的作用，可减轻或抑制紫外线对胸腺细胞和脾细胞的氧化损伤，并且呈剂量依赖性，扇贝多肽在0.5%-10.0%的浓度范围内，其抗氧化能力随浓度的增高而增强，在正常条件下可显著增强免疫细胞的活性，并且可拮抗雌激素对免疫细胞的抑制作用。提示扇贝多肽不仅具有抗氧化损伤作用，而且具有免疫增强使用。
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| 关 键 词： | 扇贝多肽 免疫细胞 紫外线 抗氧化损伤作用 海洋药物 海洋生物活性 |
| 收稿时间： | 2000/4/25 0:00:00 |
| 修稿时间： | 2000年4月25日 |
EFFECT OF POLYPEPTIDES FROM CHLAMYS FARRERI ON THE PROLIFERATION OF IMMUNE CELLS AND ITS PROTECTION AGAINST ULTRAVIOLET IN VITRO |
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| LIU Xiao-Ping,WANG Yu-Zhen,HAN Yan-Tao,YU Ye-Jun,WANG Yue-Jun and WANG Chun-Bo.EFFECT OF POLYPEPTIDES FROM CHLAMYS FARRERI ON THE PROLIFERATION OF IMMUNE CELLS AND ITS PROTECTION AGAINST ULTRAVIOLET IN VITRO[J].Oceanologia Et Limnologia Sinica,2001,32(4):414-419. |
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| Authors: | LIU Xiao-Ping WANG Yu-Zhen HAN Yan-Tao YU Ye-Jun WANG Yue-Jun and WANG Chun-Bo |
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| Institution: | Medical College of Qingdao University,Qingdao,266021;Medical College of Qingdao University,Qingdao,266021;Medical College of Qingdao University,Qingdao,266021;Medical College of Qingdao University,Qingdao,266021;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,266071;Medical College of Qingdao University,Qingdao,266021 |
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| Abstract: | The polypeptides isolated from Chlamys farreri (PCF) by modern bioengineering technique were a water soluble small molecular with biological activity. In this paper the effects of PCF on the proliferative activities of thymocytes and splenocytes and the protective effects of PCF on those immune cells under Ultraviolet (UV) oxidative damage were detected by MTT colorometry. The results showed that PCF could reduce the ultraviolet (UV) damage on the thymocytes and splenocytes with dose dependence. In the 0.5%_10.0% concentration range the higher concentration of PCF the stronger protective effects of it. PCF could also significantly increase the proliferative activities of the thymocytes and splenocytes and could decrease or inhibit the immune suppressive effects of Estradiol on the thymocytes and splenocytes. The results indicate that PCF not only has the effect of antioxidative damage of UV irradiation but also has the effect of increasing immune function. |
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| Keywords: | Polypeptides from Chlamys farreri(PCF) Immune cells Ultraviolet damage |
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