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舟山渔场及其相邻赤潮高发区麻痹性贝类毒素研究
引用本文:胡颢琰,唐静亮,黄备,毛宏跃,王婕妤,魏娜.舟山渔场及其相邻赤潮高发区麻痹性贝类毒素研究[J].海洋与湖沼,2008,39(5):475-481.
作者姓名:胡颢琰  唐静亮  黄备  毛宏跃  王婕妤  魏娜
作者单位:浙江省舟山海洋牛态环境监测站,舟山,316000
基金项目:国家自然科学基金重大项目 , 浙江省环境保护局科研资助项目  
摘    要:采用小白鼠生物检测法和高效液相色谱荧光检测法,对采自舟山渔场及其相邻赤潮高发区贝类的麻痹性贝毒素进行了调查分析与研究。结果表明,浙江舟山海域有毒贝类检出率为7.0%。利用高效液相色谱荧光检测法对2006年5月份采集的六横的西格织纹螺、岱山的毛蚶组织提取液进行麻痹性贝毒素测定,结果显示,六横的西格织纹螺体内麻痹性贝毒素含量为0.178Mu/g,毒力值为0.0356μg STXeq/g,其毒素成分由GTX2、GTX3、STX、dcSTX、C2组成:岱山的毛蚶体内麻痹性贝毒素含量为3.494Mu/g,毒力值为0.6987μg STXeq/g,其毒素成分由STX、NEO、C1、C2组成。浙江中、南部海域有毒贝类检出率为2.2%,利用高效液相色谱荧光检测法对2006年10月份在南麂列岛采集的棒锥螺的组织提取液进行麻痹性贝毒素的测定,结果显示棒锥螺体内麻痹性贝毒素含量为0.705Mu/g,毒力值为0.1409μg STXeq/g,其毒素成分有GTX1、GTX2、GTX3、NEO、C1、C2组成。

关 键 词:麻痹性贝毒素  赤潮  小白鼠生物检测法  高效液相色谱荧光检测法  舟山渔场
收稿时间:2007/8/25 0:00:00
修稿时间:2007/10/27 0:00:00

THE SHELLFISH-BEARING PSP TOXIN IN AREAS OF HIGH RED TIDE OCCURRENCE OF THE ZHOUSHAN FISHING GROUND
HU Hao-Yan,TANG Jing-Liang,HUANG Bei,MAO Hong-Yue,WANG Jie-Yu and WEI Na.THE SHELLFISH-BEARING PSP TOXIN IN AREAS OF HIGH RED TIDE OCCURRENCE OF THE ZHOUSHAN FISHING GROUND[J].Oceanologia Et Limnologia Sinica,2008,39(5):475-481.
Authors:HU Hao-Yan  TANG Jing-Liang  HUANG Bei  MAO Hong-Yue  WANG Jie-Yu and WEI Na
Institution:Zhoushan Marine Ecological Environment Monitoring Station, Zhejiang Province, Zhoushan, 316000;Zhoushan Marine Ecological Environment Monitoring Station, Zhejiang Province, Zhoushan, 316000;Zhoushan Marine Ecological Environment Monitoring Station, Zhejiang Province, Zhoushan, 316000;Zhoushan Marine Ecological Environment Monitoring Station, Zhejiang Province, Zhoushan, 316000;Zhoushan Marine Ecological Environment Monitoring Station, Zhejiang Province, Zhoushan, 316000;Zhoushan Marine Ecological Environment Monitoring Station, Zhejiang Province, Zhoushan, 316000
Abstract:PSP(paralytic shellfish poisoning) toxin is a biological toxin generated in dinoflagellates-often the members of red tide, and is accumulated in marine shellfish body.12 artificial and wild shellfish species were sampled in the Zhou-shan Fishing Ground of the Zhoushan Archipelago, China, a major seafood production base with frequent red-tide out-breaks, and adjacent regions in May to October 2006, to determine PSP toxicity levels in shellfish by AOAC bioassay and HPLC-FD.The results show that 7.0% of the samples were PSP-contaminated in Zhoushan area and 2.2% in offshore mid-dle and southern Zhejiang.The HPLC-FD profile show that, in Nassarius siquinjorensis the concentration of PSP toxin was 0.178Mu/g, including major compositions of GTX2, GTX3, STX, dcSTX, and C2;in Scapharca subcrenata it was 3.494Mu/g, containing STX, NEO, C1, and C2;in Turritella bacillum it was 0.705Mu/g, consisting of GXT1, GXT2, GXT3, NEO, C1, and C2.
Keywords:PSP  Harmful algal bloom  Mouse bioassay  HPLC-FD  Zhoushan Fishing Ground
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