| 饲料维生素C对美国红鱼(Sciaenops ocellatus)组织NO含量、NOS活力和nNOS基因表达的影响 |
| |
| 引用本文： | 周立斌,白 茹,马细兰,王树齐,张海发.饲料维生素C对美国红鱼(Sciaenops ocellatus)组织NO含量、NOS活力和nNOS基因表达的影响[J].海洋与湖沼,2014,45(5):1122-1126. |
| |
| 作者姓名： | 周立斌 白 茹 马细兰 王树齐 张海发 |
| |
| 作者单位： | 惠州学院生命科学系 生物技术研究所 惠州 516007;汕头大学 广东省海洋生物技术重点实验室 汕头 515063;华南师范大学生命科学学院 广州 510631;惠州学院生命科学系 生物技术研究所 惠州 516007;汕头大学 广东省海洋生物技术重点实验室 汕头 515063;广东省海洋渔业试验中心 惠州 516081 |
| |
| 基金项目： | 广东省高等学校高层次人才项目, 粤财字[2013]246 号; 广东省海洋生物技术重点实验室开发基金资助项目, GPKLMB201202 号; 国家自然科学基金项目, 31201978 号; 惠州市科技计划项目, 2011B040010003 号; 惠州学院自然科学研究重点项目,2012ZD07 号 |
| |
| 摘 要： | 本试验旨在研究饲料维生素C对美国红鱼(Sciaenops ocellatus)组织中的NO含量、NOS活力和NOS基因表达的影响。采用单因子试验设计,将360尾平均体重为(147.28±7.69)g的美国红鱼随机分为6个处理,每个处理设3个重复,每个重复20尾,分别饲喂6种不同维生素C水平(0、49.3、98.9、197.5、396.4和1989.8mg/kg)的等氮等能饲料,进行为期8周的生长试验。结果表明:头肾、肝、肠、胸腺NO含量随着饲料维生素C含量增加有显著增加;脾中各组NO含量无显著性差异;脑中NO含量C0组与其它各组相比有显著性差异。头肾、肝、胸腺NOS活力随着饲料维生素C含量增加有显著增加;脾和肠中各组NOS活力无显著性差异;脑中NOS活力C396.4组与其它各组相比有显著性差异。脑中各组nNOS基因mRNA的表达含量无显著性差异;在饲料维生素C含量达到1989.8mg/kg时,其它各组织内的nNOS基因mRNA的表达量最高。综上所述,饲料中添加适当维生素C对美国红鱼组织中的NO含量、NOS活力和nNOS基因mRNA的表达有显著影响。
|
| 关 键 词： | 美国红鱼 维生素C 一氧化氮 免疫 |
| 收稿时间： | 2013/11/7 0:00:00 |
| 修稿时间： | 2014/3/19 0:00:00 |
EFFECTS OF DIETARY VITAMIN C ON NITRIC OXIDE, NITRIC OXIDE SYNTHASE ACTIVITY AND NITRIC OXIDE SYNTHASE GENE EXPRESSION IN TISSUE OF RED DRUM SCIAENOPS OCELLATUS |
| |
| ZHOU Li-Bin,BAI-Ru,MA Xi-Lan,WANG Shu-Qi and ZHANG Hai-Fa.EFFECTS OF DIETARY VITAMIN C ON NITRIC OXIDE, NITRIC OXIDE SYNTHASE ACTIVITY AND NITRIC OXIDE SYNTHASE GENE EXPRESSION IN TISSUE OF RED DRUM SCIAENOPS OCELLATUS[J].Oceanologia Et Limnologia Sinica,2014,45(5):1122-1126. |
| |
| Authors: | ZHOU Li-Bin BAI-Ru MA Xi-Lan WANG Shu-Qi and ZHANG Hai-Fa |
| |
| Institution: | Department of Life Science, Institute of Biotechnology, Huizhou University, Huizhou 516007, China; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China;College of Life Science, South China Normal University, Guangzhou 510631, China;Department of Life Science, Institute of Biotechnology, Huizhou University, Huizhou 516007, China;Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China;Guangdong Marine Fishery Development Center, Huizhou 516081, China |
| |
| Abstract: | We conducted an experiment to study the effects of dietary vitamin C on nitric oxide, NOS activity and nNOS mRNA expression in tissues of red drum Sciaenops ocellatus. Six iso-nitrogenous and iso-energetic diets were formulated to contain graded level of vitamin C (0, 49.3, 98.9, 198.7, 398.6, and 1987.4 mg/kg diet, respectively ) and fed to 18 floating net cages (1.5?1.0?1.0 m) each containing 20 fish (initial weight: 147g) twice daily (07:30, 16:30) for 8 weeks. Results indicate that after 8-week feeding trial, the levels of nitric oxide (NO) content and nitric oxide synthase (NOS) activity in head, kidney, liver, and thymus were boosted with increasing dietary vitamin C from 0 to 1987.4 mg/kg (P<0.05), but in spleen and intestines, no significant difference was found among dietary treatments. The NOS activity reached a peak value in brain fed at 398.6 mg/kg of dietary vitamin C. No significant difference in nNOS mRNA expression in brain was found among dietary treatments; the level of nNOS mRNA expression was found maximal in all tissues except for brain when fed 1987.4 mg/kg of dietary vitamin C. Therefore, adding dietary vitamin A in feed a proper amount could increase remarkably the NO content, NOS activity, and nNOS mRNA expression of red drum. |
| |
| Keywords: | red drum Sciaenops ocellatus vitamin C nitric oxide immunity |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 |
|
