| 凡纳滨对虾(Litopenaeus vannamei)病原副溶血弧菌(Vibrio parahaemolyticus)的表型及分子特征 |
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| 引用本文： | 张晓君,陈翠珍,阎斌伦,房 海,秦国民,徐 静.凡纳滨对虾(Litopenaeus vannamei)病原副溶血弧菌(Vibrio parahaemolyticus)的表型及分子特征[J].海洋与湖沼,2009,40(5):654-662. |
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| 作者姓名： | 张晓君 陈翠珍 阎斌伦 房 海 秦国民 徐 静 |
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| 作者单位： | 1. 淮海工学院海洋学院江苏省海洋生物技术重点建设实验室,连云港,222005
2. 河北科技师范学院动物科学系,秦皇岛,066600 |
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| 基金项目： | 淮海工学院江苏省海洋生物技术重点建设实验室研究基金资助,2008HS016号;;连云港市科技攻关项目资助,CN0826号;;淮海工学院引进人才科研启动基金资助项目,KQ07106号 |
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| 摘 要： | 采用常规的形态及生理生化特性检验及分子生物学等方法,对引起凡纳滨对虾幼虾发生毁灭性死亡的病原菌进行了致病性、表型生物学及分子生物学的系统研究。结果表明,分离菌为弧菌属(Vibrio Pacini 1954)的副溶血弧菌(Vibrio parahaemolyticus),对凡纳滨对虾、中国对虾及日本对虾仔虾均有强致病性。分离鉴定的4株副溶血弧菌具有淀粉酶、明胶酶、卵磷脂酶活性,但不具有蛋白酶、脂肪酶活性,KP溶血均呈阴性,且均具有K抗原;代表菌株(JGB080708-1株)的16S rRNA基因序列(长度为1456bp,GenBank登录号为GQ205448)和gyrB基因序列(长度为1195bp,GenBank登录号为GQ205453)与副溶血弧菌的两种基因序列相似性均可达98%以上。病原菌的耐药性检测结果显示,对供试49种抗菌药物中的林可霉素等6种药物耐药。
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| 关 键 词： | 凡纳滨对虾 副溶血弧菌 16S rRNA基因 gyrB基因 |
| 收稿时间： | 2008/9/15 0:00:00 |
| 修稿时间： | 2008/11/23 0:00:00 |
PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF PATHOGENIC VIBRIO PARAHAEMOLYTICUS ISOLATED FROM PENAEUS VANNAMEI |
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| ZHANG Xiao-Jun,CHEN Cui-Zhen,YAN Bin-Lun,FANG Hai,QIN Guo-Min and XU Jing.PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF PATHOGENIC VIBRIO PARAHAEMOLYTICUS ISOLATED FROM PENAEUS VANNAMEI[J].Oceanologia Et Limnologia Sinica,2009,40(5):654-662. |
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| Authors: | ZHANG Xiao-Jun CHEN Cui-Zhen YAN Bin-Lun FANG Hai QIN Guo-Min and XU Jing |
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| Institution: | 1.Key Laboratory of Oceanic Biotechnology of Jiangsu;Huaihai Institute of Technology;Lianyungang;222005;2.Department of Animal Science;Hebei Normal University of Science and Technology;Qinhuangdao;066600 |
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| Abstract: | A mass mortality of cultured Litopenaeus vannamei broke out during summer in a local farm in northern Jiangsu Province.Syndromes include swimming near water surface and the pond walls,with black gill and opaque muscle.Tissue samples were taken in the liver,and strong pathogenicity to L.vannamei,P.japonicus and P.chinensis were determined by immersion.The phenotypic characteristics of isolates(4 strains) were examined in morphology,physiology and biochemistry;the 16S rRNA and gyrB were amplified by PCR and c... |
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| Keywords: | Litopenaeus vannamei Vibrio parahaemolyticus 16S rRNA gene gyrB gene |
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