| 中国对虾(Fenneropenaeus chinensis) “黄海2号”人工感染WSSV的荧光定量分析 |
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| 引用本文： | 逄锦菲,孔 杰,孟宪红,栾 生,罗 坤,曹宝祥,刘 宁.中国对虾(Fenneropenaeus chinensis) “黄海2号”人工感染WSSV的荧光定量分析[J].海洋与湖沼,2013,44(3):782-787. |
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| 作者姓名： | 逄锦菲 孔 杰 孟宪红 栾 生 罗 坤 曹宝祥 刘 宁 |
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| 作者单位： | 1. 上海海洋大学水产与生命学院 上海 200090;中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室 青岛 266071
2. 中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室 青岛 266071 |
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| 基金项目： | 国家自然科学基金资助项目, 31072206 号, 31172402 号; 青岛市关键技术攻关类项目, 11-1-1-11-hy 号 |
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| 摘 要： | 采用人工投喂的方法对人工选育的104个"黄海2号"中国对虾(Fenneropenaeus chinensis)家系的5557尾个体体重(2.1±0.7)g]进行WSSV感染实验,分别选取存活时间最长(342h)和存活时间最短(45h)的48尾个体,定义为抗病组和敏感组。用荧光定量PCR方法检测两组对虾体内WSSV含量,结果表明,抗病组和敏感组对虾体内WSSV含量范围分别为(2.64×104)—(3.13×106)和23.2—1.92×106copies/ngDNA,平均值分别为8.71×105和3.19×105copies/ng DNA,抗病组对虾WSSV含量显著高于敏感组(P<0.05),显示抗病组比敏感组具有更强的抗病性能。对感染测试的所有对虾的死亡情况进行统计分析,结果表明,其死亡曲线不符合数量性状的正态分布特征,提示中国对虾对WSSV的抗性可能由少数几个主基因决定。本研究可为揭示中国对虾抗WSSV性状的基因决定机制提供依据。
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| 关 键 词： | 中国对虾 WSSV 人工感染 荧光定量PCR |
| 收稿时间： | 2012/7/28 0:00:00 |
| 修稿时间： | 2012/9/23 0:00:00 |
REAL-TIME PCR ASSAY FOR QUANTIFYING WSSV LOAD IN FENNEROPENAEUS CHINENSIS "HUANGHAI No.2" |
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| PANG Jin-Fei,KONG Jie,MENG Xian-Hong,LUAN Sheng,LUO Kun,CAO Bao-Xiang and LIU Ning.REAL-TIME PCR ASSAY FOR QUANTIFYING WSSV LOAD IN FENNEROPENAEUS CHINENSIS "HUANGHAI No.2"[J].Oceanologia Et Limnologia Sinica,2013,44(3):782-787. |
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| Authors: | PANG Jin-Fei KONG Jie MENG Xian-Hong LUAN Sheng LUO Kun CAO Bao-Xiang and LIU Ning |
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| Institution: | College of Aqua-life Science and Technology, Shanghai Ocean University; Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
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| Abstract: | |
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| Keywords: | Fenneropenaeus chinensis WSSV artificial infection Real-time PCR |
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