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| 来自大黄鱼(Pseudosciaena crocea)肠道的弧菌拮抗菌的筛选与鉴定 | |
| 引用本文： | 王 娟,封永辉,蔡立胜,王 建,曲宪成,张庆华.来自大黄鱼(Pseudosciaena crocea)肠道的弧菌拮抗菌的筛选与鉴定[J].海洋与湖沼,2010,41(5):707-713. |
| 作者姓名： | 王 娟 封永辉 蔡立胜 王 建 曲宪成 张庆华 |
| 作者单位： | 1. 康那香企业(上海)有限公司 2. 上海海洋大学水产与生命学院,省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306 3. 浙江大学宁波理工学院,宁波,315100 4. 上海市动物疫病预防控制中心,上海,201103 |
| 基金项目： | 上海市教委科研创新项目, 08YZ116号; 上海市重点学科建设项目, Y1101号 |
| 摘 要： | 采用点种法并以副溶血弧菌(Vp1.2164)和溶藻弧菌(Va-Y)为弧菌指示菌,对从健康的大黄鱼肠道内分离的115株细菌进行体外拮抗试验。并对筛选出的拮抗菌进行抗菌谱测定,最后利用VITEK-32细菌快速鉴定系统和16SrRNA基因序列对拮抗菌进行鉴定。结果表明,从中筛选到3株有较强拮抗作用的菌株,编号分别为X1402-1、X1108和X3914,且菌株X1402-1的抗菌作用最强,抗菌谱最广,21株病原指示菌中,对16株均能产生较强的抑制作用,其中对Vp-1.2164的抑菌直径最大,达到28.5mm,对Va-Y的抑菌直径为8.7mm;其次为菌株X1108和X3914,对两种指示弧菌的抑菌直径分别为:12.0mm、9.0mm和0mm、7.2mm。经VITEK-32细菌快速鉴定系统鉴定,3株拮抗菌分别与铜绿假单胞菌,恶臭假单胞菌,地衣芽孢杆菌的相似性均为99%。通过16SrRNA基因序列分析表明,3株拮抗菌分别与类产碱假单胞菌Pseudomonas pseudoalcaligenes(EU419918)、恶臭假单胞菌Pseudomon asputida(FJ440105)和地衣芽孢杆菌Bacillus licheniformis(DQ988136)的16SrRNA基因同源性最高,分别为100.0%、97.5%、99.0%。综合分析试验结果,X1402-1、X1108和X3914分别被确定为类产碱假单胞菌、恶臭假单胞菌和地衣芽孢杆菌。 |
| 关 键 词： | 点种法 拮抗作用 抗菌谱 筛选 细菌鉴定 |
| 收稿时间： | 2009/9/24 0:00:00 |
| 修稿时间： | 2009/11/23 0:00:00 |
SCREENING AND IDENTIFICATION OF VIBRIO-ANTAGONISTIC BACTERIA FROM THE GASTROINTESTINAL TRACT OF PSEUDOSCIAENA CROCEA |
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| WANG Juan,FENG Yong-Hui,CAI Li-Sheng,WANG Jian,QU Xian-Cheng and ZHANG Qing-Hua.SCREENING AND IDENTIFICATION OF VIBRIO-ANTAGONISTIC BACTERIA FROM THE GASTROINTESTINAL TRACT OF PSEUDOSCIAENA CROCEA[J].Oceanologia Et Limnologia Sinica,2010,41(5):707-713. | |
| Authors: | WANG Juan FENG Yong-Hui CAI Li-Sheng WANG Jian QU Xian-Cheng and ZHANG Qing-Hua |
| Institution: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University;Ningbo Institute of Technology, Zhejiang University;Shanghai Animal Disease Control Centre;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University |
| Abstract: | One hundred and fifteen strains of bacteria were isolated from the gastrointestinal tract of cultured, healthy Pseudosciaena crocea and examined for their antagonistic activities against pathogenic Vibrio parahaemolyticus (Vp-1.2164) and Vibrio alginolyticus (Va-Y) by the dot-inoculating method. Three strains of bacteria (X1402-1, X1108 and X3914) inhibited the growth of the pathogenic vibrios. Strain X1402-1 showed the strongest antagonistic activity, with the diameters of inhibition zones of Vp-1.2164 and Va-Y being 28.5mm and 8.7mm, respectively. The diameters of inhibition zones of Vp-1.2164 and Va-Y were 12.0 and 9.0mm, respectively, for strain of X1108, and 0 and 7.2mm, respectively for strain X3914. In addition, their antibacterial spectra were studied. Strain X1402-1 showed the widest antibacterial spectra, inhibiting the growth of 16 out of the 21 strains of bacteria tested. Furthermore, identification of X1402-1, X1108 and X3914 was performed by two methods. With the VITEK-32 Bacteria Identification System (Biomerieux Company), X1402-1, X1108 and X3914 were recognized as Pseudomonas aeruginosa, Pseudomonas putida, and Bacillus licheniformis, respectively; their similarities were 99%. Complementally, the results of 16S rRNA gene sequence analysis showed that nucleotide homology of these three antagonistic strains to Pseudomonas pseudoalcaligenes (EU419918), Pseudomonas putida (FJ440105), and Bacillus licheniformis (DQ988136) were 100.0%, 97.5% and 99.0%, respectively. In summary, strains of X1402-1, X1108 and X3914 were concluded to be Pseudomonas pseudoalcaligenes, Pseudomonas putida and Bacillus licheniformis, respectively. |
| Keywords: | Dot-inoculating method Antagonism Antibacterial spectra Screening Bacteria identification |
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