| 中国沿海部分地区半褶织纹螺的毒性及毒素组成分析 |
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| 引用本文： | 罗璇,于仁成,王晓杰,周名江.中国沿海部分地区半褶织纹螺的毒性及毒素组成分析[J].海洋与湖沼,2016,47(2):327-335. |
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| 作者姓名： | 罗璇 于仁成 王晓杰 周名江 |
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| 作者单位： | 中国科学院海洋研究所海洋生态与环境科学重点实验室 青岛 266071;中国科学院大学 北京 100049,中国科学院海洋研究所海洋生态与环境科学重点实验室 青岛 266071;青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室 青岛 266071,上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室 上海 201306,中国科学院海洋研究所海洋生态与环境科学重点实验室 青岛 266071 |
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| 基金项目： | 国家自然科学基金面上项目, 30571426号; 国家自然科学基金委-山东省联合基金项目, U14066403号, U14066403号; 国家海洋局公益项目, 201305010-1号; 国家自然科学基金创新研究群体项目, 40821004号。 |
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| 摘 要： | 为确定中国沿海不同地区织纹螺中的毒素成分,本研究在江苏省、浙江省和福建省设立了多个采样点,对采集的半褶织纹螺样品进行了毒性测试和毒素组成分析。研究结果显示,采集的织纹螺样品毒性范围为2—664MU(mouse unit)/g湿重,其中,采自江苏省连云港地区的织纹螺样品毒性远高于其它地区。应用高效液相色谱-质谱联用(high-performance liquid chromatography-mass spectrometry,LC-MS)方法对样品中的毒素成分进行分析,在各地的织纹螺样品中均检测到了河豚毒素(TTX)及trideoxy TTX、4-epi TTX和anhydro TTX等衍生物,但各种毒素成分所占的比例存在差异。自江苏省连云港市和浙江省舟山市采集的织纹螺样品中,Trideoxy TTX是主要的毒素成分,TTX和其它两种毒素成分次之;而自江苏省盐城市、浙江省宁波市及福建省宁德市和莆田市采集的织纹螺样品中,TTX的含量最高。采集自江苏省连云港和浙江省舟山市的大部分织纹螺样品中,肌肉组织的毒素含量最高,其次是消化腺组织,其余组织中的毒素含量最低。高毒性成分TTX的最高值主要出现在肌肉组织或消化腺组织中。从实验结果可以看出,各地的有毒织纹螺中毒素成分基本一致,而各种毒素成分所占的比例有一定的差异,织纹螺的毒性与高毒性成分TTX的含量有关。
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| 关 键 词： | 织纹螺(Nassarius spp.) 河豚毒素及其同系物 毒素组成 LC-MS |
| 收稿时间： | 2015/11/20 0:00:00 |
| 修稿时间： | 2015/12/25 0:00:00 |
TOXICITY AND TOXIN COMPOSITION OF MARINE GASTROPOD NASSARIUS SEMIPLICATUS FROM DIFFERENT REGIONS ALONG THE COAST OF CHINA |
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| LUO Xuan,YU Ren-Cheng,WANG Xiao-Jie and ZHOU Ming-Jiang.TOXICITY AND TOXIN COMPOSITION OF MARINE GASTROPOD NASSARIUS SEMIPLICATUS FROM DIFFERENT REGIONS ALONG THE COAST OF CHINA[J].Oceanologia Et Limnologia Sinica,2016,47(2):327-335. |
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| Authors: | LUO Xuan YU Ren-Cheng WANG Xiao-Jie and ZHOU Ming-Jiang |
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| Institution: | Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Oceanography Laboratory, Qingdao 266071, China,The Key Laboratory of Aquatic Genetic Resources Excavation and Utilization Conjunctive Administrated by Ministry of Education and Shanghai Ocean University, Shanghai 201306, China and Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
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| Abstract: | Marine gastropod Nassarius spp. Contains toxin causing many poisoning incidents along the coast of China. Tetrodotoxin (TTX) and its derivatives have been rewcognized in some toxic nassariids. However, little information is available on the toxin composition in nassariids collected along the coast of China. We studied the toxicity and toxin composition of Nassarius semiplicatus from the samples collected in different zones along coast of China, in Jiangsu, Zhejiang, and Fujian Provinces. The toxicity of the nassariid samples ranged 2-664 Mouse Units (MU) per gram tissue (wet weight). Toxicity of the samples from Lianyungang (Jiangsu) was much higher than that of others. In addition, we analyzed the toxin component and composition in high performance liquid chromatography-mass spectrometry (LC-MS). TTX and its derivatives, including trideoxyTTX, 4-epiTTX, and anhydroTTX were detected in all the samples, but the composition of TTXs varied among samples of different regions. TrideoxyTTX was the major component of the samples from Lianyungang and Zhoushan (Zhejiang), followed by TTX and two other derivatives. The samples from other regions, including Yancheng (Jiangsu), Ningbo (Zhejiang), and Ningde and Putian (Fujian), contained high proportion of TTX. The total toxin content in the most samples of Lianyungang and Ningbo was the highest in muscle, followed by digestive gland and the rest parts. The peak value of TTX, with high toxicity, mainly appeared in muscle or digestive gland. These findings suggested that toxic nassariids from these Chinese coastal zones had uniform toxin components, whereas their composition varied geographically. High toxicTTX was likely relative to the toxicity of nassariids. |
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| Keywords: | Nassarius spp tetrodotoxin and its derivatives toxin composition LC-MS |
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