| 兔抗对虾白斑症病毒(WSSV)独特型抗体的制备及其特性分析 |
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| 引用本文： | 韦秀梅,绳秀珍,唐小千,邢 婧,战文斌.兔抗对虾白斑症病毒(WSSV)独特型抗体的制备及其特性分析[J].海洋与湖沼,2010,41(2):286-292. |
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| 作者姓名： | 韦秀梅 绳秀珍 唐小千 邢 婧 战文斌 |
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| 作者单位： | 中国海洋大学海水养殖教育部重点实验室,青岛,266003 |
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| 基金项目： | 国家重点基础研究发展计划“973”,2006CB101806号;;国家高技术研究发展计划“863”,2006AA100312号 |
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| 摘 要： | 以前期已制备的抗WSSV囊膜蛋白单克隆抗体4G9(Ab1)杂交瘤细胞生产小鼠腹水,腹水经辛酸-硫酸铵法、ProteinG亲和层析法纯化后,用以制备兔抗血清,所得血清经纯化得到粗提的兔抗WSSV独特型抗体(Ab2)。采用竞争酶联免疫吸附实验、间接免疫荧光法、斑点免疫印迹和蛋白免疫印迹等实验方法分析了Ab2特性,结果表明:Ab2能识别Ab1并与WSSV竞争Ab1的抗原结合位点,是具有模拟WSSV特性的抗独特型抗体;Ab2能与中国对虾血细胞结合,并能部分阻断WSSV与血细胞膜的结合;其与中国对虾血细胞膜上结合的蛋白分子量分别为94.5、51.5和27.0kDa,由此推断,这三个蛋白为WSSV在中国对虾血细胞膜上的结合蛋白。本研究结果可为进一步研究WSSV侵染机理提供资料。
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| 关 键 词： | 独特型抗体 对虾白斑症病毒 竞争酶联免疫吸附实验 Dot-blot 间接免疫荧光法 Western-blot |
| 收稿时间： | 2009/2/12 0:00:00 |
| 修稿时间： | 2009/4/26 0:00:00 |
PRODUCTION AND CHARACTERIZATION OF RABBIT ANTI-IDIOTYPIC ANTIBODY OF WHITE SPOT SYNDROME VIRUS (WSSV) |
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| WEI Xiu-Mei,SHENG Xiu-Zhen,TANG Xiao-Qian,XING Jing and ZHAN Wen-Bin.PRODUCTION AND CHARACTERIZATION OF RABBIT ANTI-IDIOTYPIC ANTIBODY OF WHITE SPOT SYNDROME VIRUS (WSSV)[J].Oceanologia Et Limnologia Sinica,2010,41(2):286-292. |
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| Authors: | WEI Xiu-Mei SHENG Xiu-Zhen TANG Xiao-Qian XING Jing and ZHAN Wen-Bin |
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| Institution: | Laboratory of Pathology and Immunology of Aquatic Animals, Laboratory of Mariculture Ministry of Education of China (LMMEC), Ocean University of China;Laboratory of Pathology and Immunology of Aquatic Animals, Laboratory of Mariculture Ministry of Education of China (LMMEC), Ocean University of China;Laboratory of Pathology and Immunology of Aquatic Animals, Laboratory of Mariculture Ministry of Education of China (LMMEC), Ocean University of China;Laboratory of Pathology and Immunology of Aquatic Animals, Laboratory of Mariculture Ministry of Education of China (LMMEC), Ocean University of China;Laboratory of Pathology and Immunology of Aquatic Animals, Laboratory of Mariculture Ministry of Education of China (LMMEC), Ocean University of China |
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| Abstract: | During the past more than ten years since a white spot syndrome virus (WSSV) disease outbreak in 1993, many research papers have been published on the pathogen, pathology, detection and diagnostic methods of the disease. Recent works focused mainly on the infection mechanism and searching the strategies of prophylaxis and control of WSSV infection. The purpose of this work was to produce rabbit anti-idiotypic antibody of WSSV (Ab2), analyze its characteriza-tion, and find a new way to study WSSV infection m... |
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| Keywords: | Idiotypic antibody WSSV ELISA Dot-blot IIFA Western-blot |
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