| 海洋微藻脂肪酸组成的比较研究 |
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| 引用本文： | 李荷芳,周汉秋.海洋微藻脂肪酸组成的比较研究[J].海洋与湖沼,1999,30(1):34-40. |
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| 作者姓名： | 李荷芳 周汉秋 |
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| 作者单位： | 中国科学院海洋研究所，中国科学院海洋研究所 青岛，266071，青岛，266071 |
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| 基金项目： | 国家自然科学基金!39570084，“九五”国家重点科技项目!96-916-04-03 |
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| 摘 要： | 于１９９５￣１９９７年在中国科学院海洋研究所培养２０种海洋微藻，采用Ｂｌｉｇｈ－Ｄｙｅｒ法和气相色谱法进行脂肪酸组成的比较研究。结果表明，除个别藻外，海洋微藻的总脂含量均超过其干重的１０％，每一纲藻种都有其特征脂肪酸或几种脂肪酸的组合作为其化学分类的标记：绿藻纲中除小球藻的２０：５（ｎ－３）含量较高以外，一般含有较高的１６：４（ｎ－３），不含２２：６（ｎ－３）；硅藻纲的１６：１（ｎ－７）含量高于１
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| 关 键 词： | 海洋微藻 化学分类学 脂肪酸 微藻 |
| 收稿时间： | 1997/8/20 0:00:00 |
| 修稿时间： | 1998/9/25 0:00:00 |
COMPAPATIVE STUDIES ON FATTY ACID COMPOSTION OF MARINE MICROALGAE |
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| LI He-fang and ZHOU Han-qiu.COMPAPATIVE STUDIES ON FATTY ACID COMPOSTION OF MARINE MICROALGAE[J].Oceanologia Et Limnologia Sinica,1999,30(1):34-40. |
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| Authors: | LI He-fang and ZHOU Han-qiu |
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| Institution: | Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071;Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071 |
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| Abstract: | Total lipid content and fatty acid composition of 20 species of marine microalgaebelonging to Chlorophyceae, Baci1lariophyceae, Chrysophyceae, Dinophyceae, Rhodophyceae, culturedunder comparable conditions were studied from l995 to l997. Lipids of mdrine whcroalgae wereextracted by the method of Bligh and ryer. Total lipid content was detendned by gravimetry after1iPid exmet evaPOrahon under reduced presare. Methyl esters of fatty acid (FAME) were obtalnedusing the method of Carreau and Dubacq and purified with layer chromotograptiy of FAME,Performed on a GC 588OA instrument supplied with an electronic integfator. The results suggest thatthe total lipid contents of the microalgae were more than 10% on dry weight excephng 3 species.Each class of Ancroalgae is charaterized by a specific fatty acid profile. The specific features ofChlorophyceae were the concentlahons of 16;4(n-3) and none of the species contained 22;6(n-3)except Chlrella, which did not contrin l6;4(n-3), but contrined high concentrahon of 2O;5(n-3). Thereliable marks of Bacillarioptiyceae were a preference of 16;1(n-7) over 16:0, and high levels of14;0, 20;5(n-3). The specific featlires of Chrysophyceae were 14:0, 18:1(n-9), l8:4(n-3) and 22;6(n-3). The main indicators of autotrophic ixnophyceae were the presence of the unusual 18:5(n-3),and the high content of 22;6(n-3). The Rhodophyceae exhibited a charateristic fatty acid profiledominated by 16:0, 20;4(n-6) and 2O;5(n-3). A dishnchve feature of was class was the high contentof 20:4(n-6), which is a minor compornent in other classes. |
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| Keywords: | Marine microalgae Chemotaxonomy Fatty acids |
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