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赤潮发生机理研究──海洋原甲藻的氮营养生理特征
引用本文:黄晓航,史冬梅,张京浦,刘海航,吴超元.赤潮发生机理研究──海洋原甲藻的氮营养生理特征[J].海洋与湖沼,1997,28(1):33-38.
作者姓名:黄晓航  史冬梅  张京浦  刘海航  吴超元
作者单位:中国科学院海洋研究所!青岛,266071
基金项目:国家自然科学基金!9389008
摘    要:于1991年1月-1991年12月,以不同浓度的硝态氮(20,60,200,600μmol/L)研究对赤潮生物海洋原甲藻的生长和生化组成的影响,及其营养生理特性,结果表明,在氮浓度为200和600μmol/L时,表现光合作用强,细胞生长快,指数生长期长,叶绿素含量高,提示了由原甲藻爆发引起的赤潮与海洋富营养化有直接关系,结果还表明,海洋原甲藻可以累积蛋白质和碳水化合物,当外界氮源不能满足生长需要时

关 键 词:甲藻  氮营养生理  硝酸氮  海洋  红潮
收稿时间:3/6/1995 12:00:00 AM
修稿时间:3/5/1996 12:00:00 AM

STUDY ON NITROGEN METABOLISM OF PROROCENIRUM MICANS WITH SPECIAL REFERENCE TO THE OCCURRENCE OF RED TIDE
Huang Xiaohang,Shi Dongmei,Zhang Jingpu,Liu Haihang and Wu Chaoyuan.STUDY ON NITROGEN METABOLISM OF PROROCENIRUM MICANS WITH SPECIAL REFERENCE TO THE OCCURRENCE OF RED TIDE[J].Oceanologia Et Limnologia Sinica,1997,28(1):33-38.
Authors:Huang Xiaohang  Shi Dongmei  Zhang Jingpu  Liu Haihang and Wu Chaoyuan
Institution:Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
Abstract:The physiological aspcts of nitrogen nutrition of Prorocentrum micans were studied in the algal physiological section of the Institute of Oceanology from Jan.through Dec., 1991.Seawater was filtered, sterilized and modified by addition of KH2PO4`, FeC6H5O7 vitamin B, and K at 4mg /L, 0.5mg /L, l00ng /L and O.5Pg /L,respectively. The algae were cultured in modified seawater of different concentrations (20, 60, 200 and 600umol /L) of nitrate- Cultures Were grown under irradianee of ca.500pE /(m2.s) and temperature of 221-During the experiment, the growth, apparent photosynthesis, nitrate absorption rate, contents of chlorophyll a, crude protein and carbohydrates were measured periodically. The results were as follows. In all nitrate-nitrogen enriched culture media (20, 60, 200 and 600umol /L), longer exponential growth periods, higher contents of chlorophyll a and higher apparent photosynthetic rates were found, suggsting a positive corelation of eutrophication with dinoflagellates caused red tide- P micans absorbed more nitrate at night (16:00- 18:00) and On the contrary more carbohydrates were found in the cells at daytime. ms was because more carbohydrates were metabolized with nitrogen at night. Part of the proteins so formed was stored in the cell' In the case of nitrogen starvation, the exponential growth could be maintained up to 6 days by the consumption of stored proteins.
Keywords:Prorocentrum micans  Red tide  Nitrogen nutrition  Nitrate
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