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| 丹江口水库赤眼鳟(Squaliobarbus curriculus)遗传多样性的RAPD和ISSR分析 | |
| 引用本文： | 杨太有,关建义,陈宏喜,汪亚平.丹江口水库赤眼鳟(Squaliobarbus curriculus)遗传多样性的RAPD和ISSR分析[J].海洋与湖沼,2008,39(2):157-161. |
| 作者姓名： | 杨太有 关建义 陈宏喜 汪亚平 |
| 作者单位： | 1. 河南师范大学生命科学学院,新乡,453007 2. 河南师范大学生命科学学院,新乡,453007;新乡医学院生命科学技术系,新乡,453003 3. 中国科学院水生生物研究所,武汉,430072 |
| 基金项目： | 河南省科技攻关项目 , 河南省重点学科建设项目 |
| 摘 要： | 利用RAPD和ISSR两种分子标记技术,分析了丹江口水库野生赤眼鳟30个个体的遗传多样性。用11个RAPD引物对其基因组DNA进行扩增,共获得101个重复性好且谱带清晰的扩增位点,片段大小在100—3000bp之间,其中多态性位点63个,多态位点比例为62.38%;个体间遗传距离在0.1049—0.3417之间,平均为0.1742。用10个ISSR引物共检测到88个位点,其中多态性位点61个,多态位点比例为69.32%;个体间遗传距离在0.1088—0.3847之间,平均为0.1907。结果显示,丹江口水库野生赤眼鳟群体的多态位点比例和平均遗传距离均较大,说明其有较高的遗传多样性。 |
| 关 键 词： | 赤眼鳟 遗传多样性 RAPD ISSR |
| 修稿时间： | 2006年12月28 |
GENETIC DIVERSITY IN RAPD AND ISSR ON SQUALIOBARBUS CURRICULUS IN DANJIANGKOU RESERVOIR |
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| YANG Tai-You,GUAN Jian-Yi,CHEN Hong-Xi,WANG Ya-Ping.GENETIC DIVERSITY IN RAPD AND ISSR ON SQUALIOBARBUS CURRICULUS IN DANJIANGKOU RESERVOIR[J].Oceanologia Et Limnologia Sinica,2008,39(2):157-161. | |
| Authors: | YANG Tai-You GUAN Jian-Yi CHEN Hong-Xi WANG Ya-Ping |
| Abstract: | Two types of molecular markers, Randomly Amplified Polymorphic DNA (RAPD) and Inter-Simple Se- quence Repeat (ISSR) were used to detect the genetic diversities among 30 wild individuals of Squaliobarbus curriculus collected from Danjiangkou Reservoir, Hubei of China in August 2006. Using 11 RAPD primers screened from 40primers, a total of 101 reproducible and stable bands, ranging from 100 to 3000bp, were amplified from 30 individuals. 63 of 101 loci detected were polymorphic, of which polymorphic loci took 62.38%. Genetic distance among the individuals was from 0.1049 to 0.3417, averaged at 0.1742. Using 10 ISSR primers selected from 77 primers, 88 reproducible and stable bands were observed, ranging from 100 to 3000bp, and 69.32% of them were polymorphic. The genetic distance among the indi- viduals was from 0.1088 to 0.3847, in average of 0.1907. These results show that the genetic diversification of S. curricu- lus in the reservoir was high. |
| Keywords: | Squaliobarbus curriculus Genetic diversity RAPD ISSR |
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