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宁波沿海陆源排污口蓝藻(Cyanobacteria)和裸藻纲(Euglenoidea)生物的分布特点
引用本文:谢利,王朝阳,张迪骏,周君,司开学,张红燕,韩姣姣,崔晨茜,董丽莎,苏秀榕.宁波沿海陆源排污口蓝藻(Cyanobacteria)和裸藻纲(Euglenoidea)生物的分布特点[J].海洋与湖沼,2016,47(1):188-194.
作者姓名:谢利  王朝阳  张迪骏  周君  司开学  张红燕  韩姣姣  崔晨茜  董丽莎  苏秀榕
作者单位:宁波大学海洋学院 宁波,国家海洋局北海环境监测中心,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波
基金项目:海洋公益性行业专项经费资助项目, 201105007-4号; 浙江省重大科技攻关项目, 2006C13089号; 宁波市科技局资助项目, 2008C50027号
摘    要:采用高通量454焦磷酸测序方法对宁波沿海2个重点排污口、8个一般排污口的20个站位水样进行分析,得到2011年的3月、5月、8月和10月份各排污口蓝藻和含叶绿体单细胞生物的分布情况。研究结果表明:宁波沿海陆源排污口中含叶绿体单细胞生物数量最多,占91.857%,包括眼虫门(Euglenozoa)、绿藻门(Chlorophyta)、隐藻门(Cryptophyta)生物。其中主要为眼虫门的裸藻纲(Euglenoidea)生物占90.08%。蓝藻门的集球藻目(Synechococcophycideae)和颤藻亚目(Oscillatoriophycideae)含量相对较多,分别占5.70%和1.79%;排污口中裸藻纲生物数量季节性差异比较大,3月份、8月份数量相对较多,5月份和10月份较少,推测这类生物的数量与季节性温度变化有关;各排污口污染物、这两类生物检出量和种类有一定差异,在氮磷含量高的排污口(如S9号)其检出量并不高,推测检出量与排污口的主要污染物中氨氮含量没有直接关系,可能受氮磷比的影响。

关 键 词:蓝藻    454焦磷酸测序    陆源排污口    16S  rRNA
收稿时间:2014/6/25 0:00:00
修稿时间:2015/1/28 0:00:00

CYANOBACTERIA AND EUGLENOIDEA DISTRIBUTION IN SEWAGE OUTLETS ALONG NINGBO COAST
XIE Li,WANG Zhao-Yang,ZHANG Di-Jun,ZHOU Jun,SI Kai-Xue,ZHANG Hong-Yan,HAN Jiao-Jiao,CUI Chen-Qian,DONG Li-Sha and SU Xiu-Rong.CYANOBACTERIA AND EUGLENOIDEA DISTRIBUTION IN SEWAGE OUTLETS ALONG NINGBO COAST[J].Oceanologia Et Limnologia Sinica,2016,47(1):188-194.
Authors:XIE Li  WANG Zhao-Yang  ZHANG Di-Jun  ZHOU Jun  SI Kai-Xue  ZHANG Hong-Yan  HAN Jiao-Jiao  CUI Chen-Qian  DONG Li-Sha and SU Xiu-Rong
Institution:NingBo University,Beihai Environmental Monitoring Center of the State Oceanic Administration,NingBo University,NingBo University,NingBo University
Abstract:Water samples were collected at 20 sites spread across 10 sewage outlets, two key sewage outlets, and eight general sewage outlets in Ningbo City during March, May, August, and October of 2011. Research results showed that: the Chloroplast in the largest number of export accounted for 91.857%. The amount of the Synechococcophycideae and Oscillatoriophycideae were relatively more, respectively accounting for 5.704% and 1.787%. The Chloroplast number in different season was various, relatively large in March and August, relatively small in October and May. The number of Chloroplast was related to the seasonal temperature change. There were some differences in the amount and types of pollutants in the sewage outfall. Although the sewage outfall (such as S9) exported the high nitrogen and phosphorus content, the amount of cyanobacteria detected was not high, so there was no direct relationship between the amount of cyanobacteria and the content of nitrogen and phosphorus in the sewage outlet. We speculated that the amount of cyanobacteria was affected by the ratio of nitrogen and phosphorus rather than the content of these. And different cyanobacteria species need the nitrogen and phosphorus ratio.
Keywords:Cyanobacteria    454 pyrosequencing    land-based sewage outfall    16S rRNA
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