| 应用高通量测序技术分析北方刺参养殖池塘环境菌群结构 |
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| 引用本文： | 窦妍,赵晓伟,丁君,何鹏.应用高通量测序技术分析北方刺参养殖池塘环境菌群结构[J].海洋与湖沼,2016,47(1):122-129. |
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| 作者姓名： | 窦妍 赵晓伟 丁君 何鹏 |
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| 作者单位： | 大连海洋大学农业部北方海水增养殖重点实验室,大连海洋大学农业部北方海水增养殖重点实验室,大连海洋大学农业部北方海水增养殖重点实验室,大连海洋大学农业部北方海水增养殖重点实验室 |
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| 基金项目： | 国家海洋局海洋公益项目,201105007-2号。 |
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| 摘 要： | 刺参(Apostichopus japonicus)是一种经济价值很高的海珍品,具有很高的药用价值和营养价值,本文采用高通量测序技术分析了大连地区3个养殖公司刺参养殖环境中室内养殖池塘水体、室外养殖水体和沉积物中菌群结构特征,9个样品所得有效序列为26503—37825条,可归为1502—5741个分类操作单元(OTUs)。研究结果表明:(1)刺参养殖环境中具有较高的菌群多样性,其中3个公司的刺参养殖沉积物环境中菌群多样性较其相应公司刺参养殖水环境中菌群多样性高;(2)9个样品中的细菌可归为26个门类,除常见报道的变形菌门、拟杆菌门、绿弯菌门、浮霉菌门、蓝细菌门、酸杆菌门、疣微菌门、厚壁菌门和放线菌门外,还有17个门类的鲜见报道的菌群被检测到;(3)各样品中菌群组成虽有差异,但变形菌门和拟杆菌门为优势菌,约占50.54%—99.91%。
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| 关 键 词： | 刺参 高通量测序 变形菌门 |
| 收稿时间： | 2015/3/24 0:00:00 |
| 修稿时间： | 2015/6/15 0:00:00 |
APPLICATION OF HIGH-THROUGHPUT SEQUENCING FOR ANALYZING BACTERIAL COMMUNITIES IN EARTHEN PONDS OF SEA CUCUMBER AQUACULTURE IN NORTHERN CHINA |
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| DOU Yan,ZHAO Xiao-Wei,DING Jun and HE Peng.APPLICATION OF HIGH-THROUGHPUT SEQUENCING FOR ANALYZING BACTERIAL COMMUNITIES IN EARTHEN PONDS OF SEA CUCUMBER AQUACULTURE IN NORTHERN CHINA[J].Oceanologia Et Limnologia Sinica,2016,47(1):122-129. |
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| Authors: | DOU Yan ZHAO Xiao-Wei DING Jun and HE Peng |
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| Institution: | Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China,Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China,Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China and Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China |
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| Abstract: | Sea cucumber Apostichopus japonicus has become an economically valuable holothurian species because of their anti-tumor properties and good nutritional values. We applied high-throughput sequencing to explore bacterial community in earthen aquaculture ponds of the sea cucumber in Dalian, North China. For nine samples collected, the number of effective sequences ranged 26503-37825, and they could be classified into 1502-5741 operational taxonomic units. Results show great bacterial diversities in the aquaculture. The estimated richness and diversity indices were higher in sediment samples than those in water samples. In total, 26 phyla were identified, among which Proteobacteria, Bacteroidetes, Chloroflexi, Planctomycetes, Cyanobacteria, Acidobacteria, Verrucomicrobia, Firmicutes, and Actinobacteria were commonly reported, and other 17 were firstly detected and rarely reported. Despite the differences in microbe composition, Proteobacteria and Bacteroidetes (50.54%-99.91%) were the dominant phyla in all samples. |
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| Keywords: | Sea cucumber High-throughput sequencing Proteobacteria |
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