| 抗大口黑鲈(Micropterus salmoides)蛙虹彩病毒药效模型的构建及其抗病毒中药筛选 |
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| 引用本文： | 李秋语,黄小红,郝贵杰,胡大雁,张成赛,牛晨,袁雪梅,黄雷,于喆,姚嘉赟,杨桂连,姜建湖.抗大口黑鲈(Micropterus salmoides)蛙虹彩病毒药效模型的构建及其抗病毒中药筛选[J].海洋与湖沼,2022,53(6):1513-1522. |
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| 作者姓名： | 李秋语 黄小红 郝贵杰 胡大雁 张成赛 牛晨 袁雪梅 黄雷 于喆 姚嘉赟 杨桂连 姜建湖 |
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| 作者单位： | 吉林农业大学动物科学与技术学院 吉林省动物微生态制剂工程研究中心 吉林长春 130118;浙江省淡水水产研究所 农业农村部淡水渔业健康养殖重点实验室 浙江省鱼类健康与营养重点实验室 浙江湖州 313001;湖州市农业科技发展中心 浙江湖州 313001 |
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| 基金项目： | 湖州市公益性应用研究项目,2021GZ28号,2021GZ24号,2021GZ31号,201105007-4号;国家重点研发蓝色粮仓项目,2019YFD0900104号;湖州市乡村振兴专项,2018ZD2025号。 |
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| 摘 要： | 大口黑鲈蛙虹彩病毒(LMBV)是一种严重危害大口黑鲈养殖的病害,但目前缺乏有效的防控手段。通过研究水温、鱼体大小以及攻毒剂量等条件对大口黑鲈感染蛙虹彩病毒的影响,构建体内抗病毒药效筛选模型,同时利用MTT法构建体外药物筛选模型,进而通过体外和体内药效模型,对29种中草药进行抗病毒药效评价,对筛选出药效最佳的2种中药进行体外和体内抗病毒药效评价。结果表明:水温和鱼体大小是影响体内药效模型的2个关键因子,最佳体内药效模型条件为:水温为25℃,鱼体大小20g,攻毒剂量为0.1mL 109.33 TCID50/mL。结合体内和体外药效模型结果,筛选出紫花地丁和黄连这2种中药具有较好的抗病毒效果的中草药。体内药效结果表明:黄连和地丁的添加量为0.6g/kg和1.2g/kg时,其对大口黑鲈的保护率最高均达到40%,而两者用药后病毒在肝组织的抑制率分别可达71.5%和67.0%。组织病理学结果表明,当药物使用浓度为0.6g/kg和1.2g/kg时,可有效降低因大口黑鲈蛙虹彩病毒感染所导致的肝和肾等组织的病理学损伤。上述结果表明,黄连和地丁均具有较好的抗蛙虹彩病毒的作用。
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| 关 键 词： | 大口黑鲈(Micropterus salmoides) 蛙虹彩病毒 抗病毒模型 紫花地丁 黄连 |
| 收稿时间： | 2022/3/22 0:00:00 |
| 修稿时间： | 2022/5/11 0:00:00 |
CONSTRUCTION OF EFFICACY MODEL AGAINST LARGEMOUTH BASS VIRUS AND SCREENING OF ANTIVIRAL CHINESE MEDICINE |
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| LI Qiu-Yu,HUANG Xiao-Hong,HAO Gui-Jie,HU Da-Yan,ZHANG Cheng-Sai,NIU Chen,YUAN Xue-Mei,HUANG Lei,YU Zhe,YAO Jia-Yun,YANG Gui-Lian,JIANG Jian-Hu.CONSTRUCTION OF EFFICACY MODEL AGAINST LARGEMOUTH BASS VIRUS AND SCREENING OF ANTIVIRAL CHINESE MEDICINE[J].Oceanologia Et Limnologia Sinica,2022,53(6):1513-1522. |
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| Authors: | LI Qiu-Yu HUANG Xiao-Hong HAO Gui-Jie HU Da-Yan ZHANG Cheng-Sai NIU Chen YUAN Xue-Mei HUANG Lei YU Zhe YAO Jia-Yun YANG Gui-Lian JIANG Jian-Hu |
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| Institution: | Jilin Engineering Research Center of Animal Probiotics, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;Key Laboratory of Healthy Freshwater Fisheries Aquaculture, Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory of Fish Health and Nutrition, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;Huzhou Agricultural Science and Technology Development Centre, Huzhou 313001, China |
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| Abstract: | Abstract Largemouth bass iridovirus (LMBV), can cause lethal and epidemic diseases in the cultivation of Micropterus salmoides. However, there is no effective treatment to control LMBV infection. In the present study, MTT method was used to establish the in vitro drug screening model, in vivo pharmacodynamic model was also constructed from three aspects: water temperature, fish body size and the dose of virus. In vitro and in vivo pharmacodynamic models were used to evaluate the antiviral efficacy of 29 Chinese herbs. The results showed that water temperature and fish body size were two key factors affecting the in vivo model. The optimal pharmacodynamic model conditions were showed as follows: water temperature was 25 °C, fish body size was 20 g, and the dose of LMBV was 0.1 mL 109.33 TCID50/mL. Combined with the results of in vivo and in vitro efficacy, two Chinese medicines Viola philippica and Coptis chinensis Franch showed great antiviral effects were screened from 29 Chinese herbs. When the concentration of V. philippica and C. chinensis Franch were reached up to 0.6 g/kg and 1.2 g/kg, the protective rates against LMBV were 40%, respectively. Compared with non-treated fish, the viral load of LMBV in V. philippica and C. chinensis Franch treated group was reduced by 71.5% and 67.0%. Moreover, V. philippica and C. chinensis Franch can significantly relieve pathological damage in liver and kidney. In conclusion, V. philippica and C. chinensis Franch showed a great anti-viral effective against LMBV. |
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| Keywords: | Micropterus salmoides largemouth bass virus antiviral model Viola philippica Coptis chinensis Franch |
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