| 短蛸(Octopus ocellatus)酪氨酸蛋白激酶基因的克隆及其mRNA在细菌刺激后的表达规律 |
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| 引用本文： | 张冉冉,韦秀梅,杨建敏,王卫军,刘相全,冯艳微.短蛸(Octopus ocellatus)酪氨酸蛋白激酶基因的克隆及其mRNA在细菌刺激后的表达规律[J].海洋与湖沼,2015,46(1):173-180. |
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| 作者姓名： | 张冉冉 韦秀梅 杨建敏 王卫军 刘相全 冯艳微 |
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| 作者单位： | 1. 上海海洋大学水产与生命学院 上海201306;山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006
2. 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006 |
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| 基金项目： | 浙江省重大科技专项农业重点项目, 2013C02014-3号；浙江省海洋经济和渔业新兴产业补助项目。 |
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| 摘 要： | 从前期构建的短蛸(Octopus ocellatus)c DNA文库中克隆获得了一个短蛸酪氨酸蛋白激酶(Oo Btk)基因的c DNA全长,其c DNA全长1191 bp,包括5’非编码区(UTR)259 bp,3’UTR 227 bp,开放阅读框(ORF)705 bp,编码234个氨基酸,预测理论等电点为8.50,分子量为27.7 k Da。运用实时荧光定量PCR法分析了在健康短蛸的不同组织以及鳗弧菌和藤黄微球菌刺激后血细胞中Oo Btk的表达规律,结果表明:Oo Btk在肌肉、外套膜、鳃、鳃心、系统心脏、肝胰脏、肾囊、胃、性腺和血细胞等各检测组织中均有表达,其中在肝胰脏的表达量最高;短蛸经鳗弧菌和藤黄微球菌刺激后,Oo Btk在血细胞中的表达量呈现出了明显的被诱导表达趋势,在鳗弧菌和藤黄微球菌刺激后6 h表达即增强,并分别在鳗弧菌刺激后48 h和藤黄微球菌刺激后12 h达到最高。Oo Btk参与短蛸对于鳗弧菌和藤黄微球菌刺激的应答过程。
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| 关 键 词： | 短蛸 酪氨酸蛋白激酶 实时荧光定量PCR 藤黄微球菌 鳗弧菌 |
| 收稿时间： | 2014/9/10 0:00:00 |
| 修稿时间： | 6/2/2014 12:00:00 AM |
CLONING OF A TYROSINE PROTEIN KINASE GENE FROM OCTOPUS OCELLATUS AND THE EXPRESSION PATTERN AFTER BACTERIA STIMULATION |
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| ZHANG Ran-Ran,WEI Xiu-Mei,YANG Jian-Min,WANG Wei-Jun,LIU Xiang-Quan and FENG Yan-Wei.CLONING OF A TYROSINE PROTEIN KINASE GENE FROM OCTOPUS OCELLATUS AND THE EXPRESSION PATTERN AFTER BACTERIA STIMULATION[J].Oceanologia Et Limnologia Sinica,2015,46(1):173-180. |
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| Authors: | ZHANG Ran-Ran WEI Xiu-Mei YANG Jian-Min WANG Wei-Jun LIU Xiang-Quan and FENG Yan-Wei |
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| Institution: | 1. Zhejiang Ocean University, Zhoushan 316022; 2.Fishery Technology Extension Center of Yuyao, Yuyao 315400,1. Zhejiang Ocean University, Zhoushan 316022; 2.Fishery Technology Extension Center of Yuyao, Yuyao 315400,1. Zhejiang Ocean University, Zhoushan 316022; 2.Fishery Technology Extension Center of Yuyao, Yuyao 315400,1. Zhejiang Ocean University, Zhoushan 316022; 2.Fishery Technology Extension Center of Yuyao, Yuyao 315400,1. Zhejiang Ocean University, Zhoushan 316022; 2.Fishery Technology Extension Center of Yuyao, Yuyao 315400,Zhejiang Ocean University |
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| Abstract: | Two-year-old population of Mytilus coruscus and one-year-old population of Mytilus edulis from their own cohort of cultured species in Shengsi waters as well as the hybrid mussel in the middle zone between two raft culture systems were selected as experimental subjects. With morphological variables, including shell width (SW), shell length (SL), shell height (SH), straight-line distance from umbo to ligament-end (OA), from umbo to the top of dorsal margin (OB), from umbo to the rear-most point in posterior (OC), from umbo to the foothold of SH in ventral margin (OD), from ligament-end to the top of dorsal margin (AB), from the top of dorsal margin to rear-most point in posterior (BC), and from rear-most point in posterior to the foothold of SH in ventral margin (CD). Multivariate analysis methods were used to study the effect of shell frame characteristics on body weight of three species of Mytilus. The results indicated that first, the variation coefficient of shell weight was greater than that of each shell frame characteristics for three species of Mytilus. The variation coefficients of all phenotypic traits measured in the hybrid mussel were the largest among the three, and that between M. coruscus and M. edulis were close. Second, the correlation coefficients between the shell weight and each shell frame characteristics were extremely significant (P<0.01) for three species of Mytilus. However, only for the hybrid mussel, all the correlation coefficients among shell frame characteristics were extremely significant (P<0.01). Third, according to path analysis, multiple correlation indexes between the reserved frame characteristics and shell weight for M. coruscus, M. edulis and the hybrid mussel were 0.868, 0.700, and 0.931, respectively. The corresponding orders of the direct effect from reserved frame characteristics were listed in turn: OC > SH > SW, OC > SW and SL > SH > OA. Fourth, by using multiple regression analysis, regression equations were obtained to estimate the shell weight for M. coruscus, M. edulis and the hybrid mussel, respectively. |
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| Keywords: | shell frame characteristics shell weight multiple analysis Mytilus coruscus Mytilus edulis the hybrid mussel |
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