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大菱鲆(Scophthalmus maximus)抗鳗弧菌(Vibrio anguillarum)性状的微卫星分子标记研究
引用本文:郭建丽,田岳强,马爱军,黄智慧,商晓梅,王新安,杨志,曲江波.大菱鲆(Scophthalmus maximus)抗鳗弧菌(Vibrio anguillarum)性状的微卫星分子标记研究[J].海洋与湖沼,2015,46(1):157-164.
作者姓名:郭建丽  田岳强  马爱军  黄智慧  商晓梅  王新安  杨志  曲江波
作者单位:1. 中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室 青岛市海水鱼类种子工程与生物技术重点实验室 青岛 266071
2. 中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室 青岛市海水鱼类种子工程与生物技术重点实验室 青岛 266071;上海海洋大学 上海 201306
3. 烟台开发区天源水产有限公司 烟台 264003
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目);中国科学院战略性先导科技专项(A类);国家基金委创新研究群体科学基金
摘    要:采用微卫星分子标记技术分析验证了133对微卫星分子标记,以期为大菱鲆(Scophthalmus maximus)育种提供合适的抗鳗弧菌分子标记。实验鱼经鳗弧菌(Vibrio anguillarum)感染,分为非抗病组与抗病组。对两组鱼PCR扩增出的差异条带进行个体统计,再进行微卫星位点与抗鳗弧菌的相关性分析,并分析对比了两组鱼的遗传多样性。结果表明,微卫星位点Sma-USC108、Sma-USC141的等位基因片段分别在215bp和200bp与抗鳗弧菌性状的正相关性极显著,相关系数分别达到0.363和0.407(P0.01);微卫星位点FF0911、Sma-USC62、Sma-USC279的等位基因片段分别在189bp、163bp和236bp与抗鳗弧菌性状的负相关性极显著,相关系数分别达到–0.377、–0.364和–0.363(P0.01)。经过二次验证,最终确定Sma-USC141和FF0911两个微卫星位点可指导大菱鲆抗鳗弧菌的辅助育种。两组大菱鲆的群体遗传多样性分析对比表明:抗病群体与非抗病群体的遗传变异水平较低,两个群体的遗传多样性水平相当。

关 键 词:大菱鲆  微卫星标记  鳗弧菌  遗传多样性
收稿时间:7/3/2014 12:00:00 AM
修稿时间:2014/8/15 0:00:00

THE SCREENING OF MICROSATELLITES MOLECULAR MARKERS WITH REFERENCE TO ANTI-VIBRIO ANGUILLARUM OF TURBOT SCOPHTHALMUS MAXIMUS
GUO Jian-Li,TIAN Yue-Qiang,MA Ai-Jun,HUANG Zhi-Hui,SHANG Xiao-Mei,WANG Xin-An,YANG Zhi and QU Jiang-Bo.THE SCREENING OF MICROSATELLITES MOLECULAR MARKERS WITH REFERENCE TO ANTI-VIBRIO ANGUILLARUM OF TURBOT SCOPHTHALMUS MAXIMUS[J].Oceanologia Et Limnologia Sinica,2015,46(1):157-164.
Authors:GUO Jian-Li  TIAN Yue-Qiang  MA Ai-Jun  HUANG Zhi-Hui  SHANG Xiao-Mei  WANG Xin-An  YANG Zhi and QU Jiang-Bo
Institution:Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China;Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:We explored 133 pairs of microsatellite markers included in GenBank to obtain suitable molecular markers that closely associated with anti-Vibrio anguillarum for turbot (Scophthalmus maximus L.) breeding. The experimental turbot by intraperitoneal injection with Vibrio anguillarum were divided into two groups: susceptible group and resistant group. The differential bands in the two turbot groups were selected, from which the correlation between the upper anti-Vibrio anguillarum and the microsatellite loci were analyzed, and the genetic diversity compared. The results show that alleles of Sma-USC108 and Sma-USC141 at 215bp and 200bp respectively were extremely significantly correlated with anti-Vibrio anguillarum with the Pearson correlation value of 0.363 and 0.407. On the other hand, alleles of FF0911, Sma-USC62, and Sma-USC279 at 189bp, 163bp, and 236bp, respectively, were extremely significantly negative-correlated with anti-Vibrio anguillarum, with the Pearson correlation values at -0.377, -0.364, and -0.363 (P<0.01), respectively. We verified the above result with a second trial and determined that Sma-USC141 and FF0911 is useful for further molecular assistant selection of anti-Vibrio anguillarum turbot. Comparative analysis showed that they two groups were in low genetic variation at a same-level of genetic diversity.
Keywords:Copepods    Microzooplankton    Predation    Yangtze River estuary
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