| 长牡蛎(Crassostrea gigas)Wnt4基因cDNA克隆与表达分析 |
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| 引用本文： | 杨梅,许飞,刘俊,李莉,张国范.长牡蛎(Crassostrea gigas)Wnt4基因cDNA克隆与表达分析[J].海洋与湖沼,2015,46(1):35-42. |
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| 作者姓名： | 杨梅 许飞 刘俊 李莉 张国范 |
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| 作者单位： | 1. 中国科学院海洋研究所海洋生态养殖技术国家地方联合工程实验室 青岛 266071;中国科学院大学 北京 100049
2. 中国科学院海洋研究所海洋生态养殖技术国家地方联合工程实验室 青岛 266071 |
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| 基金项目： | 国家重点基础研究发展计划(973项目),2010CB126402号;国家自然科学基金重点项目,40730845号;国家高技术研究发展计划(863项目),2012AA10A405号;贝类产业技术体系项目,CARS-48号;泰山攀登计划项目。 |
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| 摘 要： | Wnt4作为Wnt基因家族的重要成员,在动物的生长发育过程中起重要调控作用。本文利用RACE技术克隆了长牡蛎Wnt4基因c DNA全长序列,该序列全长1999bp,开放阅读框为1068bp,编码355个氨基酸,该蛋白序列与人(Homo sapiens)、沙蚕(Platynereis dumerilii)和栉孔扇贝(Chlamys farreri)Wnt4蛋白的相似性分别为44%、48%和46%。通过荧光定量RT-PCR分析长牡蛎Wnt4基因在成体不同组织和不同发育阶段的表达情况,发现长牡蛎的Wnt4基因具有广泛的组织表达特点,在所检测的多种组织中(外套膜、鳃、唇瓣、消化腺、雄性性腺、雌性性腺)均有表达,推测长牡蛎的Wnt4以信号分子的形式参与多种组织细胞的生命过程;长牡蛎个体发育过程中Wnt4基因的高表达主要集中在胚胎发育的早期(桑葚期最高,原肠胚期次之),幼贝期该基因的表达量很低,说明Wnt4基因可能在早期发育阶段参与了某些器官的形成。
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| 关 键 词： | 长牡蛎 Wnt4基因 克隆 表达分析 |
| 收稿时间： | 2014/2/27 0:00:00 |
| 修稿时间： | 2014/3/20 0:00:00 |
MOLECULAR CLONING AND EXPRESSION OF WNT4 GENE IN PACIFIC OYSTER CRASSOSTREA GIGAS |
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| YANG Mei,XU Fei,LIU Jun,LI Li and ZHANG Guo-Fan.MOLECULAR CLONING AND EXPRESSION OF WNT4 GENE IN PACIFIC OYSTER CRASSOSTREA GIGAS[J].Oceanologia Et Limnologia Sinica,2015,46(1):35-42. |
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| Authors: | YANG Mei XU Fei LIU Jun LI Li and ZHANG Guo-Fan |
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| Institution: | National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao ; University of Chinese Academy of Sciences, Beijing,National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao,National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao ; University of Chinese Academy of Sciences, Beijing,National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao,National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao |
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| Abstract: | As an important member of the Wnt gene family, Wnt4 plays a pivotal role in development processes of a variety of animals. Here, using the RACE technique, we obtained the entire cDNA sequence of Wnt4 gene in Pacific oyster (Crassostrea gigas). The full length of this gene was 1999 bp, encoded 355 amino acids. Homologous analysis of the amino acids showed that C. gigas Wnt4 was over 40% similar to most species of Wnt4 including Homo sapiens, Platynereis dumerilii and Chlamys farreri. QRT-PCR results showed C. gigas Wnt4 gene was expressed in multiple tissues (such as mantle, gills, labial palp, digestive gland, male gonad and female gonad ), which implied that C. gigas Wnt4 plays various biological roles. During different developmental stages in C. gigas, the Wnt4 mRNA expression levels was highest in the early embryogenesis (the morula stage was highest, followed by gastrula stage), and lowest in the juvenile, so we inferred C. gigas Wnt4 may be involved in the formation of certain organs in early developmental stages. |
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| Keywords: | Crassostrea gigas Wnt4 gene cloning expression analysis |
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