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温度和盐度对短节硬毛藻(Chaetomorpha brachygona Harvey)生长发育的影响研究
引用本文:丁兰平,李雯诗,谭华强,颜泽伟,黄冰心.温度和盐度对短节硬毛藻(Chaetomorpha brachygona Harvey)生长发育的影响研究[J].海洋与湖沼,2015,46(1):191-196.
作者姓名:丁兰平  李雯诗  谭华强  颜泽伟  黄冰心
作者单位:汕头大学 汕头 515063
基金项目:国家星火计划项目, 2012GA608082号;浙江省重大科技专项, 2010B06015-4号
摘    要:利用双因素实验和单藻培养等方法,对采自广东南澳岛的短节硬毛藻(Chaetomorpha brachygona Harvey)进行研究,在不同温度和盐度条件下,对该硬毛藻的生长、活力以及形态进行了观察与测定。结果表明,温度和盐度对短节硬毛藻的生长均有一定影响,当温度为20°C时,藻体培养第3、6和12天活力分别为0.70—0.75Y、0.60—0.70Y和0.55—0.65Y,培养12天时藻体有生殖细胞形成,此温度有利于藻体维持活力;温度为25—30°C时,培养第3天时会大量形成生殖细胞;在盐度10、20、40和50时,藻体的生长与温度相关,即30°C25°C20°C;在盐度20—30时,藻体长度最大达4.55 cm;在盐度10—30时,更有利于藻体生长,整体趋势为2030104050。因此,实验条件下,温度25—30°C、盐度10—30更利于短节硬毛藻的生长和形成生殖细胞。本实验表明,短节硬毛藻为暖水、广盐性种,有可能在我国南方海区大量生长并扩散,具有引发绿潮的潜力。

关 键 词:短节硬毛藻  环境因子  绿潮藻  量子产量Yield
收稿时间:9/1/2014 12:00:00 AM
修稿时间:2014/10/15 0:00:00

EFFECTS OF TEMPERATURE AND SALINITY ON GROWTH AND DEVELOPMENT OF MARINE GREEN ALGAL CHAETOMORPHA BRACHYGONA HARVEY
DING Lan-Ping,LI Wen-Shi,TAN Hua-Qiang,YAN Ze-Wei and HUANG Bing-Xin.EFFECTS OF TEMPERATURE AND SALINITY ON GROWTH AND DEVELOPMENT OF MARINE GREEN ALGAL CHAETOMORPHA BRACHYGONA HARVEY[J].Oceanologia Et Limnologia Sinica,2015,46(1):191-196.
Authors:DING Lan-Ping  LI Wen-Shi  TAN Hua-Qiang  YAN Ze-Wei and HUANG Bing-Xin
Institution:State Marine Facilities and Cultivation Engineering Research Center, Zhejiang Ocean University, Changzhi Island , Lincheng new District, Zhoushan, 316000, China,State Marine Facilities and Cultivation Engineering Research Center, Zhejiang Ocean University, Changzhi Island , Lincheng new District, Zhoushan, 316000, China,State Marine Facilities and Cultivation Engineering Research Center, Zhejiang Ocean University, Changzhi Island , Lincheng new District, Zhoushan, 316000, China,State Marine Facilities and Cultivation Engineering Research Center, Zhejiang Ocean University, Changzhi Island , Lincheng new District, Zhoushan, 316000, China
Abstract:In this study, we reported the primary sequence of Larimichthys crocea complement component C3 (L.c-C3 ) and C4 (L.c-C4 ) cDNA. L.c-C3 open reading frame (ORF) is 4962 bp and encodes for 1653 amino acids with a putative signal peptide of 23 amino acid residues. L.c-C4 open reading frame (ORF) is 5088bp and encodes for 1695aa with a putative signal peptide of 19 amino acid residues. The deduced amino acid sequence showed that L.c-C3 and L.c-C4 has conserved residues and domains known to be critical for C3 and C4 function. Phylogenetic analyses of L.c-C3 and L.c-C4 showed that L. crocea was most closely related to Miichthys miiuy. The mRNA expressions of L.c-C3 and L.c-C4 were detectable at different tissues. L.c-C3 and L.c-C4 were expressed in a wide range of adult tissues, with the highest expression levels in the liver. During the different development stages from fertilized egg to newborn larvae of the large yellow croaker the highest expression levels of L.c-C3 gene was not found, but L.c-C4 was significantly up-regulated. Bacterial challenge experiments showed that the levels of L.c-C3 and L.c-C4 mRNAs expression were up-regulated in the liver and spleen of adult large yellow croaker. The results show that L.c-C3 and L.c-C4 mRNAs expression in the large yellow croaker were influenced by bacterial stress. The results showed that L.c-C3 and L.c-C4 mRNAs expression in the large yellow croaker were influenced by bacterial stress and that L.c-C3 and L.c-C4 mRNAs may play an important role in immunity mechanisms. This study will further our understanding of the function of L.c-C3 and L.c-C4 mRNAs and the molecular mechanism of innate immunity in teleosts.
Keywords:Large  yellow croaker (Larimichthys  crocea )  Larimichthys  crocea complement  component C3 (L  c-C3)  Larimichthys  crocea complement  component C4 (L  c-C4)  Molecular  characterization  Expression  analysis
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