| 16S rDNA克隆文库解析刺参(Apostichopus japonicas)苗种培育池中生物絮团的细菌群落结构* |
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| 引用本文： | 任利华,李斌,孙国华,张秀珍,杨建敏,姜芳,刘丽娟,刘兆存.16S rDNA克隆文库解析刺参(Apostichopus japonicas)苗种培育池中生物絮团的细菌群落结构*[J].海洋与湖沼,2015,46(1). |
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| 作者姓名： | 任利华 李斌 孙国华 张秀珍 杨建敏 姜芳 刘丽娟 刘兆存 |
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| 作者单位： | (1. 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验,山东华春渔业有限公司东营 |
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| 基金项目： | 山东省农业重大应用技术创新项目(2011-2013)；山东省自然基金面上项目（ZR2013CM029）；山东省现代农业产业技术体系-刺参创新团队（SDAIT-08-011-02）；海洋经济创新发展区域示范项目（20130125）；山东省水生动物营养与饲料泰山学者岗位经费资助 |
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| 摘 要： | 提要 通过采集东营和蓬莱地区采用生物絮团技术的刺参(Apostichopus japonicas)苗种培育池(DYt和PLt)及其对照池(DYc和PLc)海水样品,构建细菌16S rDNA 克隆文库,对其中细菌群落的多样性和群落组成结构进行了分析。结果表明,四个文库Coverage值在34.7%～54.8%之间,文库丰富度指数(Chao)66.2～314.1,shannon多样性指数从3.01～4.07变动,Pielou均匀度指数0.68～0.85,样品的细菌群落均具有很高的多样性,蓬莱刺参苗种培育池细菌多样性均高于东营；生物絮团文库中细菌包括拟杆菌门(Bacteroidetes)、变形细菌门(Proteobacteria)、厚壁菌门(Firmicutes)等八个菌门和未知类群,黄杆菌群(Flavobacteria)、α-变形菌群(Alphaproteobacteria)芽孢杆菌纲(Bacilli)为主要优势菌群；DYt和PLt处理组文库细菌多样性减少并出现特征菌群,生物絮团调控技术改变了刺参苗种培育环境的微生物群落结构。生物絮团的细菌群落结构研究,为揭示生物絮团的作用机制并进一步有效利用提供研究基础和依据。
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| 关 键 词： | 16S rDNA克隆文库 细菌群落结构 生物絮团 刺参育苗池 |
ANALYSIS OF BACTERIAL COMMUNITY STRUCTURE OF BIO-FLOC FROM APOSTICHOPUS JAPONICAS BREEDING PONDS IN DIFFERENT AREAS BY 16S RDNA CLONE LIBRARY |
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| Ren Lihu,li Bin,Sun Guohu,Zhang Xiuzhen,Yang Jianmin,JIang Fang,Liu Lijuan and Liu Zhaocun.ANALYSIS OF BACTERIAL COMMUNITY STRUCTURE OF BIO-FLOC FROM APOSTICHOPUS JAPONICAS BREEDING PONDS IN DIFFERENT AREAS BY 16S RDNA CLONE LIBRARY[J].Oceanologia Et Limnologia Sinica,2015,46(1). |
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| Authors: | Ren Lihu li Bin Sun Guohu Zhang Xiuzhen Yang Jianmin JIang Fang Liu Lijuan and Liu Zhaocun |
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| Institution: | Shandong Marine Fisheries Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai, 264006,Shandong Marine Fisheries Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai, 264006,Shandong Marine Fisheries Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai, 264006,Shandong Marine Fisheries Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai, 264006,Shandong Marine Fisheries Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai, 264006,Shandong Marine Fisheries Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai, 264006,Shandong Marine Fisheries Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai, 264006,Shandong Huachun fisheries Ltd., Dongying, 257236 |
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| Abstract: | In the present study, 16S rDNA clone libraries of biofloc and the control in Apostichopus japonicas breeding ponds in Dongying and Penglai were constructed (DYt, DYc, PLt and PLc), Bacterial diversity and bacterial community structures were analyzed. The results showed that the coverage of the four libraries ranged from 34.7% to 54.8%, the richness index (Chao) was 66.2~314.1, Shannon diversity index was 3.01~4.07, and Pielou evenness index was 0.68~0.85. It was indicted that bacterial communities were diverse and bacterial diversity of sample from Apostichopus japonicas breeding pond in Penglai were higher than that in Dongying. The bacteria in biofloc library were categorized into eight Phylums, including Bacteroidetes, Proteobacteria, Firmicutes and other unknown taxes. Flavobacterium, Alphaproteobacteria and Bacilli were the main bacterial communities. The Diversity of DYt and PLt decreased and characteristic bacterial group were presented in that two libraries, it was showed that the biofloc technology had changed the microbial community structure in aquaculture environment. Study on bacterial community structure of biofloc can help to interpret the mechanism of that and provide theoretical foundation for further effectiveSutilization. |
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| Keywords: | 16S rDNA clone library bacterial community biofloc Apostichopus japonicas breeding pond |
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