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| 厚壳贻贝(Mytilus coruscus)血细胞cDNA文库的构建及部分EST 序列分析 | |
| 引用本文： | 王日昕,廖 智,刘 梅,鲁 涛,武 梅,何光源.厚壳贻贝(Mytilus coruscus)血细胞cDNA文库的构建及部分EST 序列分析[J].海洋与湖沼,2009,40(5):603-607. |
| 作者姓名： | 王日昕 廖 智 刘 梅 鲁 涛 武 梅 何光源 |
| 作者单位： | 1. 华中科技大学中英HUST-RRes基因工程和基因组学联合实验室,生命科学与技术学院,武汉,430074;浙江海洋学院海洋科学学院海洋生物资源及分子工程实验室,舟山,316004 2. 浙江海洋学院海洋科学学院海洋生物资源及分子工程实验室,舟山,316004 3. 华中科技大学中英HUST-RRes基因工程和基因组学联合实验室,生命科学与技术学院,武汉,430074 |
| 基金项目： | 浙江省科技厅面上科研农业项目,2008C22026号;;浙江省教育厅重点项目,20070430号;;浙江省科技厅新苗人才计划项目,2008R40G2110003号 |
| 摘 要： | 采用TRIZOLReagent提取厚壳贻贝血细胞总RNA,分离纯化mRNA,在此基础上以pCMV sport6质粒为载体通过Gateway技术构建了高质量的厚壳贻贝血细胞cDNA文库,以期获得厚壳贻贝血细胞的EST序列标签以及可能的免疫相关功能基因序列。从文库中随机挑选28个克隆进行序列测定及在线BLAST分析。结果表明,在28个随机克隆中,有4个克隆为rRNA基因,7个克隆为未知基因,推测是新基因,17个克隆为功能基因,包括脱氢酶,细胞色素氧化酶,热休克蛋白,金属结合蛋白,肌动蛋白等。以上研究结果为筛选厚壳贻贝免疫相关的新基因,深入研究厚壳贻贝免疫相关因子的分子多样性及免疫机制奠定了基础。 |
| 关 键 词： | 厚壳贻贝 血细胞 免疫因子 cDNA文库 EST |
| 收稿时间： | 2008/4/20 0:00:00 |
| 修稿时间： | 2008/7/30 0:00:00 |
CONSTRUCTION OF HAEMOLYMPHE cDNA LIBRARY OF MYTILUS CORUSCUS AND SCREENING OF EXPRESSED SEQUENCE TAGS |
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| WANG Ri-Xin,LIAO Zhi,LIU Mei,LU Tao,WU Mei and HE Guang-Yuan.CONSTRUCTION OF HAEMOLYMPHE cDNA LIBRARY OF MYTILUS CORUSCUS AND SCREENING OF EXPRESSED SEQUENCE TAGS[J].Oceanologia Et Limnologia Sinica,2009,40(5):603-607. | |
| Authors: | WANG Ri-Xin LIAO Zhi LIU Mei LU Tao WU Mei and HE Guang-Yuan |
| Institution: | China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zh;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology (HUST) |
| Abstract: | As one of major artificial breeding marine mollusks in South China,Mytilus coruscus is an economically important species,and its antimicrobial defense has been an attractive issue to researchers.The Immunity of M.coruscus is related to various immune molecules in plasma,of which antimicrobial peptide is a strong defensin against invasive or non-invasive bacteria,and is intact to eukaryotic cells,which makes the peptide a candidate molecular for developing new biological antibiotics.To obtain the expressed s... |
| Keywords: | Mytilus coruscus Haemolymphe Immune factor cDNA library EST |
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