| 甲基睾丸酮在尼罗罗非鱼(Oreochromis niloticus)肌肉中的代谢和消除规律研究 |
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| 引用本文： | 祝璟琳,邹芝英,杨 弘,李大宇,肖 炜,卫程亮.甲基睾丸酮在尼罗罗非鱼(Oreochromis niloticus)肌肉中的代谢和消除规律研究[J].海洋与湖沼,2012,43(5):1016-1022. |
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| 作者姓名： | 祝璟琳 邹芝英 杨 弘 李大宇 肖 炜 卫程亮 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心、农业部淡水渔业和种质资源利用重点实验室,无锡,214081 |
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| 基金项目： | 现代农业产业技术体系建设专项资金, CARS-49 号; 中央级公益性科研院所基本科研业务费专项资金项目, 2009JBFB04号; 农业部农业科研杰出人才及其创新团队项目“现代农业人才支撑计划经费”资助 |
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| 摘 要： | 在实验水温(28±2)℃条件下,以60mg/kg单剂量甲基睾丸酮对尼罗罗非鱼混饲口灌给药后,采用超高效液相色谱-串联质谱联用(UPLC-MS/MS)法测定尼罗罗非鱼肌肉中的药物水平,研究甲基睾丸酮在尼罗罗非鱼肌肉中的代谢及消除规律。结果表明,肌肉中药物浓度时间数据符合一级吸收二室开放模型,主要药代动力学参数达峰时间T(peak)为3.04h,质量浓度C(max)为33.331ng/g,消除半衰期T(1/2)β为23.073h。肌肉中甲基睾丸酮浓度24h内呈指数型下降,具有较高的消除速率,其后消除得较缓慢,消除方程符合C=46.18e0.101t。29d后罗非鱼肌肉中甲基睾丸酮水平在美国FDA的检测限0.8ng/g以下,给药120d后肌肉中检测不到甲基睾丸酮残留。
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| 关 键 词： | 超高效液相色谱-串联质谱(UPLC-MS/MS) 尼罗罗非鱼 甲基睾丸酮 代谢 消除 |
| 收稿时间： | 2011/12/21 0:00:00 |
| 修稿时间： | 2012/2/25 0:00:00 |
THE METABOLISM AND ELIMINATION OF 17a-METHYLTESTOSTERONE IN MUSCLE OF NILE TILAPIA OREOCHROMIS NILOTICUS |
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| ZHU Jing-Lin,ZOU Zhi-Ying,YANG Hong,LI Da-Yu,XIAO Wei and WEI Cheng-Liang.THE METABOLISM AND ELIMINATION OF 17a-METHYLTESTOSTERONE IN MUSCLE OF NILE TILAPIA OREOCHROMIS NILOTICUS[J].Oceanologia Et Limnologia Sinica,2012,43(5):1016-1022. |
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| Authors: | ZHU Jing-Lin ZOU Zhi-Ying YANG Hong LI Da-Yu XIAO Wei and WEI Cheng-Liang |
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| Institution: | Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences |
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| Abstract: | |
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| Keywords: | Ultro high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) Nile tilapia Oreochromis niloticus 17a-methyltestosterone Metabolism Elimination |
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