| 鱼类致病鰤鱼诺卡氏菌(Nocardia seriolae)的LAMP检测技术建立与应用 |
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| 引用本文： | 王国良,刘 璐,徐益军.鱼类致病鰤鱼诺卡氏菌(Nocardia seriolae)的LAMP检测技术建立与应用[J].海洋与湖沼,2011,42(1):27-31. |
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| 作者姓名： | 王国良 刘 璐 徐益军 |
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| 作者单位： | "应用海洋生物技术"教育部重点实验室,宁波大学生命科学与生物工程学院,宁波315211 |
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| 基金项目： | 长江学者和创新团队发展计划资助, IRT0734 号; 农业部公益性行业科研专项, 200903029 号; 浙江省自然科学基金项目, Y3080244 号 |
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| 摘 要： | 针对鲫鱼诺卡氏菌16S-23S rRNA基因内转录间隔序列,设计了四条LAMP引物,在外内引物浓度比1:8、dNTP浓度0.8-1.2mmol/L、Mg<'2>浓度10-14mmol/L、反应温度60-65℃、反应时间60min的优化条件下,扩增产物经电泳后呈现特异性的LAMP梯形条带,建立的LAMP法具有高灵敏度,达...
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| 关 键 词： | 鰤鱼诺卡氏菌 16S-23S rRNA基因 环介导恒温基因扩增检测法 |
| 收稿时间： | 2010/4/15 0:00:00 |
| 修稿时间： | 7/9/2010 12:00:00 AM |
DEVELOPMENT AND APPLICATION OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR DETECTION OF FISH PATHOGENIC NOCARDIA SERIOLAE |
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| WANG Guo-Liang,LIU Lu and XU Yi-Jun.DEVELOPMENT AND APPLICATION OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR DETECTION OF FISH PATHOGENIC NOCARDIA SERIOLAE[J].Oceanologia Et Limnologia Sinica,2011,42(1):27-31. |
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| Authors: | WANG Guo-Liang LIU Lu and XU Yi-Jun |
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| Institution: | Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University |
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