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| 应用双特异分子探针技术定性定量检测圆海链藻(Thalassiosira rotula) | |
| 引用本文： | 李秀芹,于志刚,甄 毓,蔡青松,米铁柱,李荣秀.应用双特异分子探针技术定性定量检测圆海链藻(Thalassiosira rotula)[J].海洋与湖沼,2009,40(1):12-18. |
| 作者姓名： | 李秀芹 于志刚 甄 毓 蔡青松 米铁柱 李荣秀 |
| 作者单位： | 1. 中国海洋大学生命科学与技术学部,青岛,266003 2. 中国海洋大学化学化工学院,青岛,266003 3. 中国科学院上海生命科学研究院,上海,200031 4. 中国海洋大学环境科学与工程学院,青岛,266003 5. 上海交通大学生命科学技术学院,上海,200030 |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家高技术研究发展计划(863计划)，国家自然科学基金 |
| 摘 要： | 设计了一套圆海链藻(Thalassiosira rotula)特异性探针,运用双特异分子探针技术,对圆海链藻(Thalassiosira rotula)进行了定性定量检测.结果表明,本实验中设计的一套探针与其它十几种藻无交叉反应,具有种特异性;细胞裂解液直接杂交检测优于提纯RNA样品检测;对自然样品做了初步检测,发现天然海水中的其它浮游生物对该检测方法影响很小. |
| 关 键 词： | 圆海链藻 双特异分子探针技术 赤潮藻检测 |
| 收稿时间： | 6/4/2007 12:00:00 AM |
| 修稿时间： | 5/8/2008 12:00:00 AM |
DETECTION AND QUANTIFICATION OF THALASSIOSIRA ROTULA WITH DOUBLE SPECIFIC PROBE ASSAY |
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| LI Xiu-Qin,YU Zhi-Gang,ZHEN Yu,CAI Qing-Song,MI Tie-Zhu and LI Rong-Xiu.DETECTION AND QUANTIFICATION OF THALASSIOSIRA ROTULA WITH DOUBLE SPECIFIC PROBE ASSAY[J].Oceanologia Et Limnologia Sinica,2009,40(1):12-18. | |
| Authors: | LI Xiu-Qin YU Zhi-Gang ZHEN Yu CAI Qing-Song MI Tie-Zhu and LI Rong-Xiu |
| Institution: | 1.Marine Life Sciences College;Ocean University of China;Qingdao;266003;2.Chemistry and Chemical Engineering College;3.Shanghai Institutes for Biological Sciences;Chinese Academy of Sciences;Shanghai;200031;4.Environmental Science and Engineering College;5.School of Life Science and Biotechnology;Shanghai Jiao Tong University;200030 |
| Abstract: | A double specific probe assay was applied to detect and quantify Thalassiosira rotula,a common species of harmful algae bloom,in which a set of oligonucleotide probes targeting 18S rRNA was used.The probes are species-specific,without obvious incident cross-reactivity with other species.Results of detection on the same number of cells to crude cell lysate and extracted total RNA were compared,showing that the former was better.A preliminary test was made on natural samples,and the noise produced by complex ... |
| Keywords: | Thalassiosira rotula Double specific probe assay Harmful algae detection |
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