| 金乌贼(Sepia esculenta)精子超微结构和低温损伤电镜观察 |
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| 引用本文： | 韩 松,郑小东,李 琪,于瑞海.金乌贼(Sepia esculenta)精子超微结构和低温损伤电镜观察[J].海洋与湖沼,2010,41(4):483-488. |
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| 作者姓名： | 韩 松 郑小东 李 琪 于瑞海 |
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| 作者单位： | 中国海洋大学,海水养殖教育部重点实验室,青岛,266003 |
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| 基金项目： | 国家自然科学基金项目资助，中国海洋大学海水养殖教育部重点实验室开放课题资助，国家高技术研究发展计划(863计划)资助项目 |
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| 摘 要： | 利用扫描和透射电镜技术研究了金乌贼成熟精子的超微结构,以及超低温冷冻对精子超微结构和形态的影响。结果表明,金乌贼精子为鞭毛型精子,外部附有光滑的裙边状被膜,分为头部、中部和尾部三部分。头部由顶体和细胞核组成,顶体位于细胞核前端,呈"囊"状。细胞核为长纺锤状。中部是由线粒体和垂直的中心粒构成,线粒体大小不一,多层分布。尾部细长,轴丝为典型的"9+2"结构。经超低温冷冻的精子被膜上出现很多凹陷;头部顶体破裂,大量的内容物流出,细胞核膜分离;线粒体解离,嵴变形;尾部缠绕在一起,少数鞭毛出现脱落。
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| 关 键 词： | 金乌贼 精子 超微结构 |
| 收稿时间： | 9/9/2009 12:00:00 AM |
| 修稿时间： | 1/9/2010 12:00:00 AM |
ULTRASTRUCTURE OF SEPIA ESCULENTA (CEPHALOPODA: SEPIIDA) SPERMATOZOA AND ITS DAMAGE BY LOW TEMPERATURE |
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| HAN Song,ZHENG Xiao-Dong,LI Qi and YU Rui-Hai.ULTRASTRUCTURE OF SEPIA ESCULENTA (CEPHALOPODA: SEPIIDA) SPERMATOZOA AND ITS DAMAGE BY LOW TEMPERATURE[J].Oceanologia Et Limnologia Sinica,2010,41(4):483-488. |
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| Authors: | HAN Song ZHENG Xiao-Dong LI Qi and YU Rui-Hai |
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| Institution: | The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China;The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China;The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China;The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China |
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| Abstract: | Electron microscopy was used to study the morphologies and ultrastructures of the spermatozoa of golden cuttlefish Sepia esculenta before and after low temperature treatment.The flagellum-like sperm of S.esculenta,covered by a smooth membrane,consisted of a head,a middle piece and a tail.The head comprised a bubble-shaped acrosome and an ellipse nucleus,with the acrosome located in the front of the nucleus.The middle piece contained many mitochondria and vertical central granules.The mitochondria were uneve... |
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| Keywords: | Sepia esculenta Spermatozoon Ultrastructure |
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