| 翘嘴鲌(Culter alburnus)TMEM-57基因的克隆、组织表达和单核苷酸多态性分析 |
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| 引用本文： | 祁鹏志,郭宝英,谢从新,吴常文,陆诗敏,段友健.翘嘴鲌(Culter alburnus)TMEM-57基因的克隆、组织表达和单核苷酸多态性分析[J].海洋与湖沼,2012,43(6):1196-1201. |
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| 作者姓名： | 祁鹏志 郭宝英 谢从新 吴常文 陆诗敏 段友健 |
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| 作者单位： | 1. 华中农业大学水产学院,武汉,430070
2. 浙江海洋学院国家海洋设施养殖工程技术研究中心,舟山,316004 |
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| 基金项目： | 国家科技支撑计划, 2012BAD25B06 号 |
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| 摘 要： | 采用RT-PCR和快速扩增cDNA末端(rapid amplification of cDNA ends,RACE)技术首次克隆了翘嘴鲌TMEM-57蛋白基因的cDNA全序列,该序列全长为2822bp,其中5′-UTR区93bp,3′-UTR区731bp,开放阅读框(open reading frame,ORF)为1998bp,编码665个氨基酸。NJ法系统进化分析将同科或属中TMEM-57基因分成一个分支,表现出种属间的高度保守性。利用荧光定量PCR(qPCR)技术检测了不同组织的表达量,发现在卵巢中表达量最高,其次为心脏和脑。用直接测序法对TMEM-57基因部分区段单核苷酸多态性(SNP)进行了检测,寻找到11个新的SNP位点。
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| 关 键 词： | 翘嘴鲌 TMEM-57 荧光定量PCR 单核苷酸多态性 |
| 收稿时间： | 2012/1/17 0:00:00 |
| 修稿时间： | 3/5/2012 12:00:00 AM |
CLONING, TISSUE EXPRESSION AND SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF CULTER ALBURNUS TMEM-57 |
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| QI Peng-Zh,GUO Bao-Ying,XIE Cong-Xin,WU Chang-Wen,LU Shi-Min and DUAN You-Jian.CLONING, TISSUE EXPRESSION AND SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF CULTER ALBURNUS TMEM-57[J].Oceanologia Et Limnologia Sinica,2012,43(6):1196-1201. |
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| Authors: | QI Peng-Zh GUO Bao-Ying XIE Cong-Xin WU Chang-Wen LU Shi-Min and DUAN You-Jian |
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| Institution: | College of Fisheries, Huazhong Agricultural University;National Research Centre of Mariculture Equipments and Engineering, Technology, Marine Science College of Zhejiang Ocean University;College of Fisheries, Huazhong Agricultural University;National Research Centre of Mariculture Equipments and Engineering, Technology, Marine Science College of Zhejiang Ocean University;College of Fisheries, Huazhong Agricultural University;College of Fisheries, Huazhong Agricultural University |
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| Abstract: | |
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| Keywords: | Culter alburnus TMEM-57 Real-time fluorescence quantitative PCR Single nucleotide polymorphism |
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