| 5种海水鱼同工酶表达的组织特性及其电泳的初步分析 |
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| 引用本文： | 王可玲,尤锋,徐成,吴谡琦.5种海水鱼同工酶表达的组织特性及其电泳的初步分析[J].海洋与湖沼,1996,27(6):626-631. |
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| 作者姓名： | 王可玲 尤锋 徐成 吴谡琦 |
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| 作者单位： | 中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071 |
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| 基金项目： | 国家科委攀登计划B项目!PDB6－5－2 |
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| 摘 要： | 于１９９４年７－１２月，山东，福建省沿海采集真鲷、黑鲷、牙鲆、鲈和小黄鱼等５种黄６９２个生化样品，应用水平淀粉胶和垂直聚丙烯酰胺凝胶电泳方法，对５种鱼的肌肉，眼睛、心脏、鳍，鳃、肾脏共７种组织的２３种同工酶进行了初步分析。
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| 关 键 词： | 同工酶 电泳 组织特性 海水鱼 真鲷 牙鲆 |
| 收稿时间： | 9/5/1995 12:00:00 AM |
| 修稿时间： | 1996/8/13 0:00:00 |
THE SPECIFIC EXPRESSION OF ISOZYMES IN DIFFERENT TISSUES AND PRELIMINARY BIOCHEMICAL GENETIC ANALYSIS BASED ON THE ELECTROPHORESIS OF FIVE MARINE FISHES |
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| Wang Keling,You Feng,Xu Cheng and Wu Suqi.THE SPECIFIC EXPRESSION OF ISOZYMES IN DIFFERENT TISSUES AND PRELIMINARY BIOCHEMICAL GENETIC ANALYSIS BASED ON THE ELECTROPHORESIS OF FIVE MARINE FISHES[J].Oceanologia Et Limnologia Sinica,1996,27(6):626-631. |
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| Authors: | Wang Keling You Feng Xu Cheng and Wu Suqi |
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| Institution: | Institute of Oceanology, Chinese Academy of sciences, Qingdao 266071;Institute of Oceanology, Chinese Academy of sciences, Qingdao 266071;Institute of Oceanology, Chinese Academy of sciences, Qingdao 266071;Institute of Oceanology, Chinese Academy of sciences, Qingdao 266071 |
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| Abstract: | Sixty-three fishes belonging to 5 different species (Pagrusomus major, Sparus macrocephalus, Paralichthys olivaceus, Lateolabrax Japonicus, Pseudosciaena polyactis) were collected from the coastal waters of Shandong and Fujian provinces from July to December in 1994. Biochemical samples were extracted by homogenizing from seven tissues (muscle,eye, liver, heart, gill, fin and kidney) of each species separately. Horizontal starch gel and ventical polyacrylamide gel electrophoresis were used in examining 692 samples. Four kinds of buffer systems, TC1, TC2 and EBT for starch gel and TG for polyacrylamide gel electrophoresis, were tested. Altogether 22 isozymes and a prowin were examined through different kinds of hssue or different kinds of buffer systems. only EST, Pm, and SOD were electrophoresed by polyaCrydride ge, the others by hawh gel The results showed the isozymes expedon was twhiy hssue specific. Among the buffer systems tested, TC1, EBT and TG, seemed very useful for the future applicahon due to thdr good resoinhon of isozymes and a protrin, Which eAnbital stable expression and high level of adivity in four kinds of hssue whthe, eye, liver and hcart) during the forphotals (Tab. 1).Furthennre, two buffer systems supporting the stareh ge electrophoresis gave good resolu tion of 12 isozymes: TC1 to LDH, MDH, IDHP and MEP; EBT to ADH, SDH,G6PDH, CAT, GDH, PGM, PGDH, and GPI. Another buffer sydri, TG Was a superior to EST, Pm and SOD for polyacrylamide gel electrophoresis (Tab.2, Tab.3). Finally, based on the zymograms, five isozymes, e.g. LDH, IDHP, ADH, GDH and EST (Plate I), were analynd genhcally on thdr subunit structure, coding gene led and nUmbers as Well as thdr specific expression in there five marine fishes. |
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| Keywords: | Isozyme Electrophoresis Tissue specificity Marine fishes |
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